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Strain Name:
C57BL/6J-MtgxR9554Btlr/Mmmh
Stock Number:
069350-MU
Citation ID:
RRID:MMRRC_069350-MU
Other Names:
R9554 (G1)
Major Collection:

Strain Information

Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Kif15
Name: kinesin family member 15
Synonyms: N-10 kinesin, HKLP2, 3930402I10Rik, 3110023M17Rik, Knsl7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Pid1
Name: phosphotyrosine interaction domain containing 1
Synonyms: 5033414K04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98496
Homologene: 9924
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 60,251,128 bp (GRCm38)
  • A to G, chromosome 1 at 64,714,591 bp (GRCm38)
  • A to G, chromosome 1 at 84,159,311 bp (GRCm38)
  • A to T, chromosome 1 at 139,740,431 bp (GRCm38)
  • T to A, chromosome 2 at 69,431,153 bp (GRCm38)
  • T to A, chromosome 2 at 76,717,349 bp (GRCm38)
  • C to A, chromosome 2 at 89,590,366 bp (GRCm38)
  • T to A, chromosome 2 at 101,688,608 bp (GRCm38)
  • T to A, chromosome 2 at 181,240,677 bp (GRCm38)
  • A to G, chromosome 3 at 93,773,033 bp (GRCm38)
  • A to G, chromosome 3 at 96,581,721 bp (GRCm38)
  • A to T, chromosome 4 at 112,391,718 bp (GRCm38)
  • A to G, chromosome 5 at 32,964,732 bp (GRCm38)
  • T to A, chromosome 5 at 66,676,306 bp (GRCm38)
  • T to C, chromosome 5 at 136,224,152 bp (GRCm38)
  • C to T, chromosome 6 at 39,399,904 bp (GRCm38)
  • C to T, chromosome 6 at 87,085,341 bp (GRCm38)
  • G to A, chromosome 7 at 27,155,292 bp (GRCm38)
  • T to A, chromosome 7 at 27,703,039 bp (GRCm38)
  • T to C, chromosome 7 at 92,858,352 bp (GRCm38)
  • A to T, chromosome 7 at 102,984,567 bp (GRCm38)
  • C to A, chromosome 7 at 105,389,708 bp (GRCm38)
  • A to G, chromosome 7 at 108,395,158 bp (GRCm38)
  • T to C, chromosome 7 at 110,441,464 bp (GRCm38)
  • T to C, chromosome 7 at 125,467,822 bp (GRCm38)
  • T to C, chromosome 7 at 127,552,405 bp (GRCm38)
  • C to A, chromosome 8 at 53,608,735 bp (GRCm38)
  • A to C, chromosome 9 at 39,258,460 bp (GRCm38)
  • T to A, chromosome 9 at 40,100,864 bp (GRCm38)
  • G to T, chromosome 9 at 122,999,520 bp (GRCm38)
  • A to G, chromosome 10 at 44,441,246 bp (GRCm38)
  • T to C, chromosome 10 at 76,496,476 bp (GRCm38)
  • G to A, chromosome 10 at 80,524,363 bp (GRCm38)
  • A to T, chromosome 10 at 129,966,312 bp (GRCm38)
  • T to A, chromosome 11 at 51,048,396 bp (GRCm38)
  • A to T, chromosome 11 at 69,360,189 bp (GRCm38)
  • T to A, chromosome 11 at 101,754,788 bp (GRCm38)
  • T to C, chromosome 11 at 110,138,281 bp (GRCm38)
  • A to T, chromosome 12 at 28,557,415 bp (GRCm38)
  • A to G, chromosome 12 at 70,169,214 bp (GRCm38)
  • G to A, chromosome 12 at 109,454,963 bp (GRCm38)
  • A to T, chromosome 13 at 22,219,819 bp (GRCm38)
  • G to A, chromosome 13 at 42,154,775 bp (GRCm38)
  • T to A, chromosome 13 at 67,390,412 bp (GRCm38)
  • A to G, chromosome 14 at 30,479,734 bp (GRCm38)
  • A to G, chromosome 14 at 51,101,519 bp (GRCm38)
  • G to A, chromosome 15 at 65,889,588 bp (GRCm38)
  • G to A, chromosome 15 at 66,913,051 bp (GRCm38)
  • A to G, chromosome 15 at 76,309,587 bp (GRCm38)
  • C to A, chromosome 16 at 3,987,956 bp (GRCm38)
  • T to C, chromosome 16 at 5,070,628 bp (GRCm38)
  • T to A, chromosome 16 at 14,200,716 bp (GRCm38)
  • G to A, chromosome 16 at 16,490,489 bp (GRCm38)
  • T to A, chromosome 16 at 19,565,972 bp (GRCm38)
  • G to A, chromosome 17 at 15,405,758 bp (GRCm38)
  • C to T, chromosome 18 at 78,783,384 bp (GRCm38)
  • G to A, chromosome 19 at 11,390,344 bp (GRCm38)
  • C to T, chromosome 19 at 46,668,726 bp (GRCm38)
  • A to T, chromosome 19 at 59,345,142 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9554 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069350-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.