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Strain Name:
C57BL/6J-MtgxR9594Btlr/Mmmh
Stock Number:
069387-MU
Citation ID:
RRID:MMRRC_069387-MU
Other Names:
R9594 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Apobec3
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Synonyms: Rfv-3, CEM15, Rfv3, Gm20117
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 80287
Homologene: 105420
Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • G to C, chromosome 1 at 30,829,922 bp (GRCm38)
  • A to G, chromosome 1 at 85,632,514 bp (GRCm38)
  • A to G, chromosome 1 at 170,840,833 bp (GRCm38)
  • T to C, chromosome 1 at 183,314,077 bp (GRCm38)
  • G to T, chromosome 2 at 27,450,361 bp (GRCm38)
  • C to T, chromosome 2 at 30,346,660 bp (GRCm38)
  • C to T, chromosome 2 at 85,579,538 bp (GRCm38)
  • T to C, chromosome 2 at 87,950,200 bp (GRCm38)
  • A to G, chromosome 2 at 101,644,356 bp (GRCm38)
  • A to T, chromosome 2 at 132,546,928 bp (GRCm38)
  • A to T, chromosome 2 at 177,193,980 bp (GRCm38)
  • A to T, chromosome 3 at 60,613,438 bp (GRCm38)
  • A to G, chromosome 3 at 98,134,573 bp (GRCm38)
  • A to G, chromosome 3 at 100,922,949 bp (GRCm38)
  • A to G, chromosome 3 at 115,090,136 bp (GRCm38)
  • T to C, chromosome 4 at 41,505,091 bp (GRCm38)
  • T to G, chromosome 4 at 43,427,329 bp (GRCm38)
  • T to C, chromosome 4 at 118,781,755 bp (GRCm38)
  • T to C, chromosome 4 at 154,894,135 bp (GRCm38)
  • C to A, chromosome 5 at 36,796,463 bp (GRCm38)
  • T to C, chromosome 5 at 120,547,956 bp (GRCm38)
  • T to C, chromosome 5 at 124,830,043 bp (GRCm38)
  • T to A, chromosome 6 at 23,025,027 bp (GRCm38)
  • A to G, chromosome 6 at 24,089,101 bp (GRCm38)
  • A to G, chromosome 6 at 41,670,839 bp (GRCm38)
  • C to T, chromosome 6 at 49,447,287 bp (GRCm38)
  • A to G, chromosome 6 at 55,385,485 bp (GRCm38)
  • G to A, chromosome 6 at 89,869,895 bp (GRCm38)
  • C to A, chromosome 6 at 124,727,765 bp (GRCm38)
  • T to C, chromosome 6 at 126,865,414 bp (GRCm38)
  • A to G, chromosome 7 at 24,972,154 bp (GRCm38)
  • T to C, chromosome 7 at 25,398,703 bp (GRCm38)
  • T to G, chromosome 7 at 108,103,456 bp (GRCm38)
  • T to C, chromosome 8 at 3,851,183 bp (GRCm38)
  • T to A, chromosome 8 at 82,990,515 bp (GRCm38)
  • C to A, chromosome 8 at 104,428,120 bp (GRCm38)
  • A to G, chromosome 9 at 51,119,260 bp (GRCm38)
  • A to G, chromosome 9 at 80,381,641 bp (GRCm38)
  • G to A, chromosome 9 at 106,647,209 bp (GRCm38)
  • T to A, chromosome 10 at 75,052,041 bp (GRCm38)
  • C to T, chromosome 10 at 79,724,725 bp (GRCm38)
  • T to A, chromosome 10 at 96,617,401 bp (GRCm38)
  • T to A, chromosome 10 at 127,502,785 bp (GRCm38)
  • T to A, chromosome 10 at 129,046,485 bp (GRCm38)
  • G to A, chromosome 11 at 8,451,142 bp (GRCm38)
  • C to T, chromosome 11 at 52,773,867 bp (GRCm38)
  • A to G, chromosome 11 at 70,228,032 bp (GRCm38)
  • T to C, chromosome 11 at 75,165,931 bp (GRCm38)
  • T to C, chromosome 11 at 97,334,901 bp (GRCm38)
  • T to C, chromosome 11 at 101,367,834 bp (GRCm38)
  • C to A, chromosome 12 at 99,393,035 bp (GRCm38)
  • A to G, chromosome 13 at 55,733,036 bp (GRCm38)
  • A to T, chromosome 14 at 13,814,959 bp (GRCm38)
  • A to G, chromosome 14 at 101,918,700 bp (GRCm38)
  • T to A, chromosome 15 at 66,735,260 bp (GRCm38)
  • T to A, chromosome 15 at 79,906,452 bp (GRCm38)
  • G to T, chromosome 16 at 17,802,609 bp (GRCm38)
  • G to T, chromosome 17 at 24,649,948 bp (GRCm38)
  • C to A, chromosome 17 at 34,899,764 bp (GRCm38)
  • A to T, chromosome 17 at 36,015,563 bp (GRCm38)
  • C to G, chromosome 17 at 37,197,417 bp (GRCm38)
  • G to T, chromosome 18 at 82,646,113 bp (GRCm38)
  • T to C, chromosome 19 at 37,287,115 bp (GRCm38)
  • T to C, chromosome 19 at 46,397,235 bp (GRCm38)
  • GTTTTTT to GTTTTTTT, chromosome X at 152,795,631 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9594 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069387-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.