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Strain Name:
C57BL/6J-MtgxR9600Btlr/Mmmh
Stock Number:
069393-MU
Citation ID:
RRID:MMRRC_069393-MU
Other Names:
R9600 (G1)
Major Collection:

Strain Information

Casq2
Name: calsequestrin 2
Synonyms: cardiac calsequestrin, ESTM52, cCSQ, Csq2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12373
HGNC: HGNC:1513
Homologene: 20330
Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66865
Homologene: 6078
Lrp5
Name: low density lipoprotein receptor-related protein 5
Synonyms: LRP7, LR3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16973
HGNC: HGNC:6697
Homologene: 1746
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 10,163,752 bp (GRCm38)
  • A to G, chromosome 1 at 21,350,980 bp (GRCm38)
  • T to C, chromosome 1 at 74,383,529 bp (GRCm38)
  • A to T, chromosome 1 at 91,961,555 bp (GRCm38)
  • T to C, chromosome 1 at 136,277,198 bp (GRCm38)
  • T to C, chromosome 2 at 26,392,586 bp (GRCm38)
  • T to C, chromosome 2 at 27,230,501 bp (GRCm38)
  • T to C, chromosome 2 at 32,811,108 bp (GRCm38)
  • C to A, chromosome 2 at 45,097,168 bp (GRCm38)
  • T to C, chromosome 2 at 70,982,039 bp (GRCm38)
  • A to T, chromosome 2 at 94,374,545 bp (GRCm38)
  • A to G, chromosome 2 at 153,054,473 bp (GRCm38)
  • A to G, chromosome 2 at 166,915,199 bp (GRCm38)
  • G to T, chromosome 3 at 27,498,792 bp (GRCm38)
  • A to T, chromosome 3 at 37,041,416 bp (GRCm38)
  • A to T, chromosome 3 at 54,194,827 bp (GRCm38)
  • A to T, chromosome 3 at 102,145,306 bp (GRCm38)
  • T to G, chromosome 3 at 152,287,549 bp (GRCm38)
  • T to A, chromosome 4 at 32,684,723 bp (GRCm38)
  • T to C, chromosome 4 at 123,471,209 bp (GRCm38)
  • G to T, chromosome 4 at 148,547,635 bp (GRCm38)
  • T to A, chromosome 5 at 38,320,831 bp (GRCm38)
  • A to C, chromosome 5 at 106,636,043 bp (GRCm38)
  • T to A, chromosome 6 at 45,992,075 bp (GRCm38)
  • A to T, chromosome 6 at 96,165,175 bp (GRCm38)
  • A to G, chromosome 6 at 114,480,492 bp (GRCm38)
  • T to A, chromosome 6 at 119,345,062 bp (GRCm38)
  • C to T, chromosome 6 at 137,837,137 bp (GRCm38)
  • T to G, chromosome 6 at 142,003,508 bp (GRCm38)
  • G to A, chromosome 6 at 142,590,376 bp (GRCm38)
  • A to G, chromosome 7 at 6,394,918 bp (GRCm38)
  • G to A, chromosome 7 at 25,000,602 bp (GRCm38)
  • C to T, chromosome 7 at 34,675,221 bp (GRCm38)
  • T to A, chromosome 7 at 44,236,851 bp (GRCm38)
  • T to A, chromosome 7 at 128,276,504 bp (GRCm38)
  • C to G, chromosome 8 at 71,290,431 bp (GRCm38)
  • T to C, chromosome 8 at 113,655,118 bp (GRCm38)
  • T to C, chromosome 8 at 124,003,434 bp (GRCm38)
  • A to G, chromosome 8 at 124,688,545 bp (GRCm38)
  • T to C, chromosome 9 at 18,655,851 bp (GRCm38)
  • G to A, chromosome 9 at 22,239,088 bp (GRCm38)
  • C to T, chromosome 9 at 66,397,312 bp (GRCm38)
  • C to A, chromosome 9 at 109,383,918 bp (GRCm38)
  • T to C, chromosome 9 at 110,986,130 bp (GRCm38)
  • T to C, chromosome 10 at 76,060,405 bp (GRCm38)
  • A to C, chromosome 11 at 16,990,119 bp (GRCm38)
  • A to T, chromosome 11 at 58,817,544 bp (GRCm38)
  • T to C, chromosome 11 at 74,393,128 bp (GRCm38)
  • A to G, chromosome 11 at 80,370,371 bp (GRCm38)
  • A to G, chromosome 11 at 83,279,222 bp (GRCm38)
  • T to C, chromosome 11 at 115,476,212 bp (GRCm38)
  • A to G, chromosome 12 at 52,886,558 bp (GRCm38)
  • T to C, chromosome 12 at 81,983,661 bp (GRCm38)
  • A to G, chromosome 12 at 103,169,713 bp (GRCm38)
  • C to T, chromosome 13 at 93,090,096 bp (GRCm38)
  • T to C, chromosome 14 at 80,006,307 bp (GRCm38)
  • T to C, chromosome 15 at 34,512,735 bp (GRCm38)
  • A to G, chromosome 17 at 21,819,880 bp (GRCm38)
  • T to C, chromosome 17 at 29,161,546 bp (GRCm38)
  • G to A, chromosome 17 at 32,486,946 bp (GRCm38)
  • T to A, chromosome 17 at 46,724,396 bp (GRCm38)
  • T to C, chromosome 18 at 12,882,220 bp (GRCm38)
  • T to C, chromosome 18 at 56,592,378 bp (GRCm38)
  • A to G, chromosome 18 at 60,300,165 bp (GRCm38)
  • C to G, chromosome 19 at 3,591,712 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9600 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069393-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.