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Strain Name:
C57BL/6J-MtgxR9614Btlr/Mmmh
Stock Number:
069407-MU
Citation ID:
RRID:MMRRC_069407-MU
Other Names:
R9614 (G1)
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Tgfa
Name: transforming growth factor alpha
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21802
Homologene: 2431
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: protein kinase U-alpha, PKUalpha, 4933403M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 24086
Homologene: 4993
Mybl2
Name: myeloblastosis oncogene-like 2
Synonyms: Bmyb, B-Myb
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17865
HGNC: HGNC:7548
Homologene: 1847
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 22,421,745 bp (GRCm38)
  • CAA to CA, chromosome 1 at 33,724,996 bp (GRCm38)
  • A to G, chromosome 1 at 162,537,200 bp (GRCm38)
  • T to A, chromosome 2 at 13,478,134 bp (GRCm38)
  • T to A, chromosome 2 at 27,989,174 bp (GRCm38)
  • A to G, chromosome 2 at 86,374,668 bp (GRCm38)
  • A to T, chromosome 2 at 125,160,525 bp (GRCm38)
  • T to A, chromosome 2 at 126,567,816 bp (GRCm38)
  • G to T, chromosome 2 at 163,064,305 bp (GRCm38)
  • C to T, chromosome 3 at 88,652,662 bp (GRCm38)
  • T to C, chromosome 4 at 3,172,587 bp (GRCm38)
  • A to C, chromosome 4 at 141,951,571 bp (GRCm38)
  • T to A, chromosome 4 at 144,196,137 bp (GRCm38)
  • A to G, chromosome 5 at 50,006,908 bp (GRCm38)
  • G to A, chromosome 5 at 86,235,520 bp (GRCm38)
  • G to A, chromosome 5 at 110,596,181 bp (GRCm38)
  • A to T, chromosome 5 at 114,013,999 bp (GRCm38)
  • C to A, chromosome 5 at 136,111,489 bp (GRCm38)
  • T to C, chromosome 5 at 143,917,602 bp (GRCm38)
  • T to TCCC, chromosome 6 at 4,756,451 bp (GRCm38)
  • A to T, chromosome 6 at 17,339,404 bp (GRCm38)
  • A to T, chromosome 6 at 85,761,726 bp (GRCm38)
  • G to A, chromosome 6 at 86,271,415 bp (GRCm38)
  • T to C, chromosome 6 at 115,619,636 bp (GRCm38)
  • A to G, chromosome 6 at 124,625,629 bp (GRCm38)
  • G to T, chromosome 7 at 12,041,839 bp (GRCm38)
  • A to T, chromosome 7 at 15,971,955 bp (GRCm38)
  • T to C, chromosome 7 at 45,615,279 bp (GRCm38)
  • T to C, chromosome 7 at 79,696,006 bp (GRCm38)
  • A to T, chromosome 7 at 80,098,177 bp (GRCm38)
  • C to T, chromosome 7 at 97,039,918 bp (GRCm38)
  • A to G, chromosome 7 at 118,541,937 bp (GRCm38)
  • A to T, chromosome 7 at 128,203,850 bp (GRCm38)
  • C to A, chromosome 8 at 16,158,225 bp (GRCm38)
  • A to C, chromosome 8 at 85,013,422 bp (GRCm38)
  • A to T, chromosome 8 at 91,260,806 bp (GRCm38)
  • T to C, chromosome 8 at 104,496,844 bp (GRCm38)
  • T to C, chromosome 8 at 125,469,826 bp (GRCm38)
  • A to T, chromosome 9 at 18,988,230 bp (GRCm38)
  • T to C, chromosome 9 at 21,993,014 bp (GRCm38)
  • G to A, chromosome 9 at 38,877,985 bp (GRCm38)
  • A to G, chromosome 9 at 57,036,721 bp (GRCm38)
  • T to A, chromosome 9 at 90,195,198 bp (GRCm38)
  • T to C, chromosome 10 at 41,721,567 bp (GRCm38)
  • C to T, chromosome 10 at 42,197,025 bp (GRCm38)
  • T to C, chromosome 10 at 53,641,048 bp (GRCm38)
  • A to G, chromosome 10 at 116,367,536 bp (GRCm38)
  • T to A, chromosome 11 at 49,535,244 bp (GRCm38)
  • T to A, chromosome 11 at 96,100,359 bp (GRCm38)
  • T to C, chromosome 11 at 97,258,351 bp (GRCm38)
  • A to G, chromosome 11 at 105,247,502 bp (GRCm38)
  • A to G, chromosome 11 at 118,393,211 bp (GRCm38)
  • T to C, chromosome 12 at 41,110,934 bp (GRCm38)
  • C to T, chromosome 13 at 28,952,096 bp (GRCm38)
  • A to T, chromosome 13 at 31,807,880 bp (GRCm38)
  • T to C, chromosome 13 at 116,305,388 bp (GRCm38)
  • A to C, chromosome 14 at 65,971,402 bp (GRCm38)
  • T to G, chromosome 14 at 76,416,543 bp (GRCm38)
  • G to A, chromosome 14 at 103,605,596 bp (GRCm38)
  • A to G, chromosome 15 at 12,375,400 bp (GRCm38)
  • A to G, chromosome 15 at 44,197,585 bp (GRCm38)
  • A to T, chromosome 15 at 58,106,723 bp (GRCm38)
  • T to C, chromosome 15 at 79,094,223 bp (GRCm38)
  • C to T, chromosome 16 at 4,288,683 bp (GRCm38)
  • A to G, chromosome 16 at 29,441,580 bp (GRCm38)
  • A to C, chromosome 16 at 30,309,575 bp (GRCm38)
  • G to A, chromosome 16 at 94,832,448 bp (GRCm38)
  • C to A, chromosome 17 at 24,982,809 bp (GRCm38)
  • C to T, chromosome 17 at 46,664,286 bp (GRCm38)
  • A to T, chromosome 17 at 87,257,993 bp (GRCm38)
  • C to T, chromosome 18 at 15,393,630 bp (GRCm38)
  • T to A, chromosome 18 at 60,300,307 bp (GRCm38)
  • C to T, chromosome 19 at 3,717,364 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9614 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069407-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.