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Strain Name:
C57BL/6J-MtgxR9627Btlr/Mmmh
Stock Number:
069420-MU
Citation ID:
RRID:MMRRC_069420-MU
Other Names:
R9627 (G1)
Major Collection:

Strain Information

Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419
Plekhj1
Name: pleckstrin homology domain containing, family J member 1
Synonyms: 9530063M10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78670
VEGA: 10
Homologene: 11389
Foxa3
Name: forkhead box A3
Synonyms: Tcf-3g, Hnf3g, Hnf-3g, Tcf3g
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15377
HGNC: HGNC:5023
Homologene: 3308
Serpinc1
Name: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
Synonyms: antithrombin, ATIII, At-3, At3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11905
HGNC: HGNC:775
Homologene: 20139
Glrx3
Name: glutaredoxin 3
Synonyms: PKC interacting cousin of thioredoxin, PICOT, Txnl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 30926
Homologene: 4769
Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66894
Homologene: 48490
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 150,630,303 bp (GRCm38)
  • A to T, chromosome 1 at 160,993,531 bp (GRCm38)
  • A to G, chromosome 1 at 171,533,417 bp (GRCm38)
  • A to G, chromosome 1 at 184,914,620 bp (GRCm38)
  • T to C, chromosome 2 at 29,144,649 bp (GRCm38)
  • T to A, chromosome 2 at 36,997,653 bp (GRCm38)
  • A to T, chromosome 2 at 68,657,490 bp (GRCm38)
  • C to A, chromosome 2 at 91,678,753 bp (GRCm38)
  • A to G, chromosome 2 at 101,955,078 bp (GRCm38)
  • AAGA to AA, chromosome 2 at 110,040,665 bp (GRCm38)
  • T to C, chromosome 3 at 93,325,928 bp (GRCm38)
  • A to G, chromosome 4 at 41,293,976 bp (GRCm38)
  • A to T, chromosome 4 at 86,388,525 bp (GRCm38)
  • A to G, chromosome 4 at 117,864,013 bp (GRCm38)
  • A to T, chromosome 4 at 121,149,805 bp (GRCm38)
  • A to C, chromosome 4 at 131,922,126 bp (GRCm38)
  • G to A, chromosome 4 at 137,613,685 bp (GRCm38)
  • C to T, chromosome 5 at 97,053,169 bp (GRCm38)
  • T to C, chromosome 5 at 98,932,010 bp (GRCm38)
  • A to G, chromosome 5 at 123,322,494 bp (GRCm38)
  • C to T, chromosome 5 at 129,864,535 bp (GRCm38)
  • T to C, chromosome 6 at 48,723,666 bp (GRCm38)
  • C to T, chromosome 6 at 115,963,313 bp (GRCm38)
  • G to A, chromosome 6 at 129,906,738 bp (GRCm38)
  • T to C, chromosome 6 at 132,777,985 bp (GRCm38)
  • T to A, chromosome 7 at 19,014,533 bp (GRCm38)
  • T to C, chromosome 7 at 120,255,307 bp (GRCm38)
  • T to A, chromosome 7 at 137,445,026 bp (GRCm38)
  • T to C, chromosome 7 at 139,228,666 bp (GRCm38)
  • T to C, chromosome 8 at 22,411,542 bp (GRCm38)
  • C to A, chromosome 8 at 70,086,870 bp (GRCm38)
  • T to A, chromosome 8 at 105,387,365 bp (GRCm38)
  • T to G, chromosome 8 at 107,552,327 bp (GRCm38)
  • T to C, chromosome 9 at 31,409,893 bp (GRCm38)
  • T to A, chromosome 9 at 62,847,021 bp (GRCm38)
  • T to C, chromosome 9 at 102,041,269 bp (GRCm38)
  • A to T, chromosome 9 at 122,137,361 bp (GRCm38)
  • T to A, chromosome 10 at 41,232,182 bp (GRCm38)
  • A to C, chromosome 10 at 80,797,660 bp (GRCm38)
  • T to C, chromosome 10 at 86,957,840 bp (GRCm38)
  • A to T, chromosome 11 at 83,069,541 bp (GRCm38)
  • C to T, chromosome 11 at 106,322,013 bp (GRCm38)
  • G to T, chromosome 12 at 13,300,202 bp (GRCm38)
  • T to C, chromosome 12 at 16,706,166 bp (GRCm38)
  • T to A, chromosome 12 at 53,104,630 bp (GRCm38)
  • A to C, chromosome 12 at 75,114,216 bp (GRCm38)
  • G to T, chromosome 13 at 23,544,623 bp (GRCm38)
  • C to A, chromosome 13 at 58,409,552 bp (GRCm38)
  • A to G, chromosome 13 at 64,917,595 bp (GRCm38)
  • G to A, chromosome 13 at 68,577,637 bp (GRCm38)
  • T to A, chromosome 13 at 100,219,830 bp (GRCm38)
  • A to G, chromosome 13 at 107,022,050 bp (GRCm38)
  • T to C, chromosome 14 at 27,472,166 bp (GRCm38)
  • T to C, chromosome 14 at 55,943,832 bp (GRCm38)
  • A to G, chromosome 14 at 69,796,900 bp (GRCm38)
  • A to T, chromosome 15 at 76,599,888 bp (GRCm38)
  • T to A, chromosome 16 at 58,889,408 bp (GRCm38)
  • A to T, chromosome 16 at 93,583,674 bp (GRCm38)
  • C to T, chromosome 17 at 6,956,928 bp (GRCm38)
  • T to A, chromosome 17 at 21,508,483 bp (GRCm38)
  • G to T, chromosome 17 at 35,235,980 bp (GRCm38)
  • A to G, chromosome 18 at 61,127,900 bp (GRCm38)
  • T to A, chromosome 19 at 11,855,546 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9627 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069420-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.