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Strain Name:
C57BL/6J-MtgxR9647Btlr/Mmmh
Stock Number:
069440-MU
Citation ID:
RRID:MMRRC_069440-MU
Other Names:
R9647 (G1)
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319582
Homologene: 35306
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Odc1
Name: ornithine decarboxylase, structural 1
Synonyms: ODC
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18263
VEGA: 12
HGNC: HGNC:8109
Homologene: 1906
Ephx2
Name: epoxide hydrolase 2, cytoplasmic
Synonyms: Eph2, sEP, sEH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13850
VEGA: 14
HGNC: HGNC:3402
Homologene: 37558
Oxsr1
Name: oxidative-stress responsive 1
Synonyms: 2810422B09Rik, 2210022N24Rik, Osr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108737
HGNC: HGNC:8508
Homologene: 31288
Ssr2
Name: signal sequence receptor, beta
Synonyms: TRAPbeta
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66256
Homologene: 2369
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 72,650,148 bp (GRCm38)
  • T to A, chromosome 1 at 90,070,536 bp (GRCm38)
  • T to C, chromosome 1 at 166,309,469 bp (GRCm38)
  • C to T, chromosome 1 at 187,322,345 bp (GRCm38)
  • C to A, chromosome 2 at 50,296,470 bp (GRCm38)
  • T to C, chromosome 2 at 71,530,132 bp (GRCm38)
  • C to A, chromosome 2 at 76,777,925 bp (GRCm38)
  • A to T, chromosome 2 at 84,694,406 bp (GRCm38)
  • T to C, chromosome 2 at 103,748,193 bp (GRCm38)
  • T to C, chromosome 2 at 126,825,642 bp (GRCm38)
  • T to A, chromosome 2 at 130,615,809 bp (GRCm38)
  • T to C, chromosome 2 at 134,639,668 bp (GRCm38)
  • T to A, chromosome 2 at 180,673,275 bp (GRCm38)
  • C to A, chromosome 2 at 181,681,191 bp (GRCm38)
  • A to C, chromosome 3 at 20,122,843 bp (GRCm38)
  • A to T, chromosome 3 at 25,434,018 bp (GRCm38)
  • T to C, chromosome 3 at 83,928,711 bp (GRCm38)
  • T to C, chromosome 3 at 88,579,899 bp (GRCm38)
  • T to A, chromosome 3 at 126,998,974 bp (GRCm38)
  • T to C, chromosome 3 at 127,540,562 bp (GRCm38)
  • C to T, chromosome 3 at 127,561,602 bp (GRCm38)
  • T to A, chromosome 3 at 154,247,733 bp (GRCm38)
  • A to C, chromosome 4 at 48,205,166 bp (GRCm38)
  • A to T, chromosome 4 at 118,146,593 bp (GRCm38)
  • T to A, chromosome 4 at 118,160,202 bp (GRCm38)
  • A to T, chromosome 4 at 141,047,024 bp (GRCm38)
  • T to G, chromosome 4 at 154,081,331 bp (GRCm38)
  • A to T, chromosome 4 at 155,464,916 bp (GRCm38)
  • C to T, chromosome 5 at 14,681,774 bp (GRCm38)
  • T to C, chromosome 5 at 25,832,410 bp (GRCm38)
  • T to A, chromosome 5 at 32,924,223 bp (GRCm38)
  • G to A, chromosome 5 at 90,472,685 bp (GRCm38)
  • G to A, chromosome 5 at 104,175,770 bp (GRCm38)
  • A to T, chromosome 5 at 150,369,519 bp (GRCm38)
  • A to G, chromosome 6 at 32,251,109 bp (GRCm38)
  • A to G, chromosome 6 at 83,102,278 bp (GRCm38)
  • A to T, chromosome 7 at 23,408,151 bp (GRCm38)
  • A to G, chromosome 7 at 29,140,492 bp (GRCm38)
  • A to G, chromosome 7 at 34,202,454 bp (GRCm38)
  • A to C, chromosome 7 at 42,708,145 bp (GRCm38)
  • T to C, chromosome 7 at 43,047,747 bp (GRCm38)
  • A to T, chromosome 7 at 43,584,178 bp (GRCm38)
  • G to T, chromosome 7 at 45,679,132 bp (GRCm38)
  • G to T, chromosome 7 at 52,013,563 bp (GRCm38)
  • G to T, chromosome 7 at 99,455,034 bp (GRCm38)
  • A to G, chromosome 7 at 140,946,830 bp (GRCm38)
  • T to A, chromosome 7 at 143,080,761 bp (GRCm38)
  • T to G, chromosome 8 at 3,155,874 bp (GRCm38)
  • T to C, chromosome 8 at 36,512,056 bp (GRCm38)
  • A to T, chromosome 8 at 71,652,238 bp (GRCm38)
  • G to T, chromosome 8 at 75,058,867 bp (GRCm38)
  • A to G, chromosome 8 at 92,840,486 bp (GRCm38)
  • C to T, chromosome 8 at 122,890,943 bp (GRCm38)
  • A to G, chromosome 9 at 14,314,703 bp (GRCm38)
  • A to T, chromosome 9 at 65,858,334 bp (GRCm38)
  • A to G, chromosome 9 at 69,348,168 bp (GRCm38)
  • A to T, chromosome 9 at 110,093,146 bp (GRCm38)
  • G to A, chromosome 9 at 119,254,866 bp (GRCm38)
  • T to A, chromosome 10 at 80,259,838 bp (GRCm38)
  • A to G, chromosome 10 at 130,112,160 bp (GRCm38)
  • A to T, chromosome 11 at 72,869,825 bp (GRCm38)
  • A to G, chromosome 11 at 73,689,774 bp (GRCm38)
  • A to T, chromosome 11 at 102,255,760 bp (GRCm38)
  • T to C, chromosome 11 at 113,677,320 bp (GRCm38)
  • T to C, chromosome 12 at 17,548,613 bp (GRCm38)
  • A to G, chromosome 12 at 76,105,101 bp (GRCm38)
  • T to A, chromosome 13 at 12,426,798 bp (GRCm38)
  • A to T, chromosome 13 at 54,719,581 bp (GRCm38)
  • A to G, chromosome 14 at 34,414,737 bp (GRCm38)
  • G to C, chromosome 14 at 50,516,095 bp (GRCm38)
  • T to C, chromosome 14 at 66,089,508 bp (GRCm38)
  • A to T, chromosome 14 at 119,018,900 bp (GRCm38)
  • A to T, chromosome 15 at 50,661,548 bp (GRCm38)
  • T to A, chromosome 15 at 76,669,260 bp (GRCm38)
  • C to A, chromosome 16 at 20,376,560 bp (GRCm38)
  • T to C, chromosome 17 at 18,243,622 bp (GRCm38)
  • A to T, chromosome 17 at 37,726,905 bp (GRCm38)
  • A to G, chromosome 17 at 67,717,175 bp (GRCm38)
  • G to A, chromosome 17 at 74,692,310 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9647 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069440-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.