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Strain Name:
C57BL/6J-MtgxR9658Btlr/Mmmh
Stock Number:
069451-MU
Citation ID:
RRID:MMRRC_069451-MU
Other Names:
R9658 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Apoa1
Name: apolipoprotein A-I
Synonyms: Apoa-1, Alp-1, Sep-1, Brp-14, Sep2, Lvtw-1, Sep-2, Ltw-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11806
HGNC: HGNC:600
Homologene: 47900
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Slc27a4
Name: solute carrier family 27 (fatty acid transporter), member 4
Synonyms: fatty acid transport protein 4, FATP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26569
Homologene: 68437
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 13,118,921 bp (GRCm38)
  • T to A, chromosome 1 at 13,701,094 bp (GRCm38)
  • T to C, chromosome 1 at 71,286,475 bp (GRCm38)
  • G to C, chromosome 1 at 73,942,023 bp (GRCm38)
  • G to T, chromosome 1 at 73,942,024 bp (GRCm38)
  • T to C, chromosome 1 at 110,061,055 bp (GRCm38)
  • A to G, chromosome 2 at 25,503,858 bp (GRCm38)
  • G to A, chromosome 2 at 29,811,289 bp (GRCm38)
  • G to A, chromosome 2 at 29,889,801 bp (GRCm38)
  • C to T, chromosome 2 at 76,885,013 bp (GRCm38)
  • C to T, chromosome 2 at 101,642,884 bp (GRCm38)
  • C to A, chromosome 2 at 112,042,478 bp (GRCm38)
  • T to C, chromosome 2 at 118,439,030 bp (GRCm38)
  • G to A, chromosome 3 at 66,264,013 bp (GRCm38)
  • T to A, chromosome 3 at 79,822,684 bp (GRCm38)
  • T to C, chromosome 3 at 90,692,774 bp (GRCm38)
  • C to T, chromosome 3 at 100,027,616 bp (GRCm38)
  • T to A, chromosome 3 at 144,837,814 bp (GRCm38)
  • T to A, chromosome 4 at 86,836,388 bp (GRCm38)
  • A to G, chromosome 4 at 98,465,140 bp (GRCm38)
  • A to T, chromosome 4 at 145,215,854 bp (GRCm38)
  • T to C, chromosome 4 at 147,986,494 bp (GRCm38)
  • T to C, chromosome 5 at 33,410,989 bp (GRCm38)
  • T to C, chromosome 5 at 117,747,360 bp (GRCm38)
  • T to C, chromosome 5 at 123,864,469 bp (GRCm38)
  • T to C, chromosome 5 at 147,543,071 bp (GRCm38)
  • G to T, chromosome 5 at 147,588,567 bp (GRCm38)
  • A to G, chromosome 6 at 40,744,377 bp (GRCm38)
  • A to C, chromosome 7 at 29,152,781 bp (GRCm38)
  • A to C, chromosome 7 at 42,820,305 bp (GRCm38)
  • A to G, chromosome 7 at 97,039,971 bp (GRCm38)
  • G to A, chromosome 7 at 103,418,182 bp (GRCm38)
  • C to T, chromosome 7 at 103,754,821 bp (GRCm38)
  • A to T, chromosome 7 at 125,707,562 bp (GRCm38)
  • G to A, chromosome 7 at 139,666,313 bp (GRCm38)
  • T to C, chromosome 8 at 9,770,114 bp (GRCm38)
  • T to C, chromosome 8 at 45,245,634 bp (GRCm38)
  • C to A, chromosome 9 at 46,229,982 bp (GRCm38)
  • T to A, chromosome 9 at 92,266,482 bp (GRCm38)
  • A to G, chromosome 9 at 104,238,529 bp (GRCm38)
  • A to T, chromosome 10 at 24,773,904 bp (GRCm38)
  • A to G, chromosome 10 at 52,090,973 bp (GRCm38)
  • T to C, chromosome 10 at 78,451,569 bp (GRCm38)
  • A to G, chromosome 10 at 112,106,963 bp (GRCm38)
  • T to A, chromosome 11 at 77,499,755 bp (GRCm38)
  • A to G, chromosome 11 at 94,372,877 bp (GRCm38)
  • C to A, chromosome 11 at 101,018,588 bp (GRCm38)
  • A to G, chromosome 11 at 104,720,294 bp (GRCm38)
  • T to C, chromosome 11 at 107,111,344 bp (GRCm38)
  • A to G, chromosome 12 at 69,192,854 bp (GRCm38)
  • A to T, chromosome 13 at 99,228,927 bp (GRCm38)
  • T to A, chromosome 13 at 99,954,246 bp (GRCm38)
  • T to A, chromosome 14 at 43,714,825 bp (GRCm38)
  • A to G, chromosome 14 at 52,622,971 bp (GRCm38)
  • T to A, chromosome 14 at 101,608,420 bp (GRCm38)
  • A to T, chromosome 15 at 9,103,361 bp (GRCm38)
  • T to A, chromosome 15 at 35,623,628 bp (GRCm38)
  • A to T, chromosome 15 at 36,152,136 bp (GRCm38)
  • C to A, chromosome 15 at 88,780,539 bp (GRCm38)
  • G to C, chromosome 15 at 94,351,745 bp (GRCm38)
  • A to T, chromosome 16 at 12,244,656 bp (GRCm38)
  • A to T, chromosome 16 at 14,140,223 bp (GRCm38)
  • T to C, chromosome 16 at 20,684,113 bp (GRCm38)
  • A to T, chromosome 16 at 59,361,743 bp (GRCm38)
  • C to A, chromosome 16 at 75,560,801 bp (GRCm38)
  • C to T, chromosome 17 at 25,080,348 bp (GRCm38)
  • C to T, chromosome 17 at 35,425,209 bp (GRCm38)
  • A to G, chromosome 17 at 64,292,873 bp (GRCm38)
  • A to T, chromosome 17 at 78,876,203 bp (GRCm38)
  • C to A, chromosome 17 at 80,653,877 bp (GRCm38)
  • A to T, chromosome 18 at 12,756,212 bp (GRCm38)
  • A to G, chromosome 18 at 31,947,368 bp (GRCm38)
  • C to T, chromosome 18 at 70,467,330 bp (GRCm38)
  • C to A, chromosome 18 at 78,109,592 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9658 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069451-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.