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Strain Name:
C57BL/6J-MtgxR9665Btlr/Mmmh
Stock Number:
069458-MU
Citation ID:
RRID:MMRRC_069458-MU
Other Names:
R9665 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, KRAB14, Zfp79, Zfp80-rs1, Staf
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20841
Homologene: 56444
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: NBL2, 4.1G, D10Ertd398e, Epb4.1l2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Mrpl35
Name: mitochondrial ribosomal protein L35
Synonyms: 1110066C01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66223
Homologene: 9598
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 24,692,984 bp (GRCm38)
  • T to C, chromosome 1 at 34,810,437 bp (GRCm38)
  • T to C, chromosome 1 at 45,803,853 bp (GRCm38)
  • A to T, chromosome 1 at 82,690,580 bp (GRCm38)
  • A to G, chromosome 1 at 87,403,735 bp (GRCm38)
  • A to G, chromosome 1 at 165,876,031 bp (GRCm38)
  • A to G, chromosome 2 at 6,046,904 bp (GRCm38)
  • A to T, chromosome 2 at 72,205,674 bp (GRCm38)
  • A to G, chromosome 2 at 76,657,483 bp (GRCm38)
  • A to T, chromosome 2 at 87,753,327 bp (GRCm38)
  • A to G, chromosome 2 at 87,974,252 bp (GRCm38)
  • A to T, chromosome 3 at 88,482,486 bp (GRCm38)
  • T to A, chromosome 4 at 43,427,554 bp (GRCm38)
  • C to T, chromosome 4 at 126,323,651 bp (GRCm38)
  • A to G, chromosome 5 at 11,042,885 bp (GRCm38)
  • A to T, chromosome 5 at 29,766,376 bp (GRCm38)
  • T to A, chromosome 5 at 30,427,551 bp (GRCm38)
  • T to C, chromosome 5 at 31,072,359 bp (GRCm38)
  • T to C, chromosome 5 at 33,217,358 bp (GRCm38)
  • T to A, chromosome 5 at 35,679,310 bp (GRCm38)
  • T to A, chromosome 5 at 73,064,956 bp (GRCm38)
  • C to A, chromosome 5 at 124,478,177 bp (GRCm38)
  • T to A, chromosome 5 at 138,173,011 bp (GRCm38)
  • T to C, chromosome 6 at 29,455,448 bp (GRCm38)
  • A to G, chromosome 6 at 71,818,720 bp (GRCm38)
  • A to G, chromosome 6 at 128,783,662 bp (GRCm38)
  • A to G, chromosome 6 at 132,737,427 bp (GRCm38)
  • T to G, chromosome 6 at 145,048,762 bp (GRCm38)
  • A to T, chromosome 7 at 7,209,794 bp (GRCm38)
  • G to T, chromosome 7 at 21,349,437 bp (GRCm38)
  • A to G, chromosome 7 at 65,312,896 bp (GRCm38)
  • C to A, chromosome 7 at 73,429,807 bp (GRCm38)
  • A to G, chromosome 7 at 83,793,371 bp (GRCm38)
  • C to G, chromosome 7 at 110,093,207 bp (GRCm38)
  • A to G, chromosome 7 at 120,045,758 bp (GRCm38)
  • A to G, chromosome 7 at 127,473,919 bp (GRCm38)
  • A to C, chromosome 7 at 142,490,405 bp (GRCm38)
  • A to G, chromosome 8 at 40,375,625 bp (GRCm38)
  • T to A, chromosome 8 at 72,674,478 bp (GRCm38)
  • G to A, chromosome 8 at 77,580,638 bp (GRCm38)
  • A to G, chromosome 8 at 79,180,127 bp (GRCm38)
  • G to A, chromosome 8 at 120,033,577 bp (GRCm38)
  • T to A, chromosome 9 at 20,213,106 bp (GRCm38)
  • T to G, chromosome 9 at 43,018,354 bp (GRCm38)
  • A to T, chromosome 9 at 67,955,743 bp (GRCm38)
  • A to G, chromosome 10 at 18,525,851 bp (GRCm38)
  • A to G, chromosome 10 at 25,441,900 bp (GRCm38)
  • G to T, chromosome 10 at 29,227,348 bp (GRCm38)
  • A to T, chromosome 10 at 117,217,438 bp (GRCm38)
  • G to T, chromosome 10 at 121,266,981 bp (GRCm38)
  • A to T, chromosome 11 at 73,954,830 bp (GRCm38)
  • A to G, chromosome 11 at 78,590,927 bp (GRCm38)
  • T to A, chromosome 11 at 110,115,454 bp (GRCm38)
  • G to T, chromosome 11 at 110,115,455 bp (GRCm38)
  • G to A, chromosome 11 at 116,232,671 bp (GRCm38)
  • A to C, chromosome 12 at 104,957,988 bp (GRCm38)
  • A to T, chromosome 12 at 113,429,826 bp (GRCm38)
  • A to T, chromosome 13 at 26,769,829 bp (GRCm38)
  • A to T, chromosome 13 at 53,285,525 bp (GRCm38)
  • T to C, chromosome 14 at 44,170,339 bp (GRCm38)
  • T to A, chromosome 14 at 63,133,468 bp (GRCm38)
  • A to G, chromosome 14 at 75,330,549 bp (GRCm38)
  • A to T, chromosome 14 at 88,469,726 bp (GRCm38)
  • T to G, chromosome 15 at 10,574,717 bp (GRCm38)
  • A to T, chromosome 15 at 55,083,441 bp (GRCm38)
  • G to T, chromosome 16 at 3,989,026 bp (GRCm38)
  • A to G, chromosome 16 at 12,267,582 bp (GRCm38)
  • T to A, chromosome 17 at 11,067,175 bp (GRCm38)
  • T to C, chromosome 17 at 12,694,140 bp (GRCm38)
  • G to T, chromosome 17 at 18,440,345 bp (GRCm38)
  • A to T, chromosome 17 at 24,977,571 bp (GRCm38)
  • A to T, chromosome 17 at 29,833,017 bp (GRCm38)
  • T to A, chromosome 17 at 34,513,661 bp (GRCm38)
  • C to T, chromosome 17 at 34,600,116 bp (GRCm38)
  • T to A, chromosome 17 at 64,834,293 bp (GRCm38)
  • A to G, chromosome 18 at 36,680,598 bp (GRCm38)
  • T to C, chromosome 19 at 9,893,965 bp (GRCm38)
  • T to C, chromosome 19 at 39,851,060 bp (GRCm38)
  • A to G, chromosome 19 at 56,831,140 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9665 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069458-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.