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Strain Name:
C57BL/6J-MtgxR9689Btlr/Mmmh
Stock Number:
069482-MU
Citation ID:
RRID:MMRRC_069482-MU
Other Names:
R9689 (G1)
Major Collection:

Strain Information

Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Spidr
Name: scaffolding protein involved in DNA repair
Synonyms: 2310008H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224008
VEGA: 16
Homologene: 51693
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217342
Homologene: 11113
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • G to A, chromosome 1 at 40,019,562 bp (GRCm38)
  • A to G, chromosome 1 at 91,546,986 bp (GRCm38)
  • A to T, chromosome 2 at 29,161,543 bp (GRCm38)
  • T to A, chromosome 2 at 112,008,779 bp (GRCm38)
  • C to T, chromosome 2 at 118,689,762 bp (GRCm38)
  • C to T, chromosome 3 at 35,976,754 bp (GRCm38)
  • T to C, chromosome 3 at 59,326,203 bp (GRCm38)
  • T to C, chromosome 3 at 97,742,525 bp (GRCm38)
  • A to T, chromosome 3 at 129,830,620 bp (GRCm38)
  • G to T, chromosome 4 at 106,671,722 bp (GRCm38)
  • A to G, chromosome 4 at 113,236,349 bp (GRCm38)
  • A to C, chromosome 4 at 118,809,802 bp (GRCm38)
  • A to T, chromosome 4 at 123,322,894 bp (GRCm38)
  • A to G, chromosome 4 at 123,471,861 bp (GRCm38)
  • A to T, chromosome 5 at 33,734,904 bp (GRCm38)
  • T to C, chromosome 5 at 72,604,827 bp (GRCm38)
  • T to A, chromosome 5 at 81,794,933 bp (GRCm38)
  • T to C, chromosome 5 at 100,787,061 bp (GRCm38)
  • T to C, chromosome 5 at 135,770,336 bp (GRCm38)
  • T to C, chromosome 6 at 18,313,650 bp (GRCm38)
  • T to A, chromosome 6 at 39,614,150 bp (GRCm38)
  • T to A, chromosome 6 at 149,328,268 bp (GRCm38)
  • A to G, chromosome 7 at 18,350,955 bp (GRCm38)
  • A to G, chromosome 7 at 119,477,294 bp (GRCm38)
  • A to G, chromosome 7 at 131,058,285 bp (GRCm38)
  • C to A, chromosome 7 at 142,175,292 bp (GRCm38)
  • T to A, chromosome 8 at 3,507,436 bp (GRCm38)
  • T to C, chromosome 8 at 23,141,237 bp (GRCm38)
  • T to A, chromosome 8 at 104,614,476 bp (GRCm38)
  • A to G, chromosome 8 at 119,445,508 bp (GRCm38)
  • A to T, chromosome 8 at 121,753,638 bp (GRCm38)
  • A to T, chromosome 9 at 39,820,505 bp (GRCm38)
  • A to T, chromosome 9 at 50,534,638 bp (GRCm38)
  • A to G, chromosome 9 at 56,474,539 bp (GRCm38)
  • G to A, chromosome 9 at 106,223,522 bp (GRCm38)
  • G to A, chromosome 10 at 77,080,744 bp (GRCm38)
  • G to A, chromosome 10 at 80,314,899 bp (GRCm38)
  • G to A, chromosome 10 at 94,199,970 bp (GRCm38)
  • A to G, chromosome 10 at 109,769,173 bp (GRCm38)
  • T to C, chromosome 11 at 73,588,860 bp (GRCm38)
  • C to A, chromosome 11 at 98,702,123 bp (GRCm38)
  • A to C, chromosome 11 at 99,024,057 bp (GRCm38)
  • T to C, chromosome 11 at 101,181,352 bp (GRCm38)
  • C to A, chromosome 11 at 116,544,813 bp (GRCm38)
  • T to C, chromosome 11 at 118,072,905 bp (GRCm38)
  • T to A, chromosome 13 at 41,141,780 bp (GRCm38)
  • A to C, chromosome 13 at 52,624,772 bp (GRCm38)
  • A to G, chromosome 13 at 56,614,287 bp (GRCm38)
  • A to G, chromosome 14 at 26,868,914 bp (GRCm38)
  • T to A, chromosome 14 at 32,337,566 bp (GRCm38)
  • T to C, chromosome 14 at 51,908,614 bp (GRCm38)
  • T to A, chromosome 14 at 58,073,223 bp (GRCm38)
  • T to C, chromosome 15 at 77,995,199 bp (GRCm38)
  • T to C, chromosome 15 at 82,112,928 bp (GRCm38)
  • T to A, chromosome 15 at 91,235,827 bp (GRCm38)
  • C to T, chromosome 15 at 96,418,314 bp (GRCm38)
  • T to C, chromosome 16 at 16,053,440 bp (GRCm38)
  • T to C, chromosome 16 at 19,699,705 bp (GRCm38)
  • C to A, chromosome 16 at 20,660,423 bp (GRCm38)
  • T to C, chromosome 16 at 23,060,474 bp (GRCm38)
  • G to T, chromosome 17 at 8,256,989 bp (GRCm38)
  • T to A, chromosome 17 at 28,067,054 bp (GRCm38)
  • A to T, chromosome 19 at 8,709,230 bp (GRCm38)
  • T to C, chromosome 19 at 11,276,694 bp (GRCm38)
  • A to T, chromosome 19 at 12,074,203 bp (GRCm38)
  • G to A, chromosome 19 at 57,381,278 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9689 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069482-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.