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Strain Name:
C57BL/6J-MtgxR9694Btlr/Mmmh
Stock Number:
069487-MU
Citation ID:
RRID:MMRRC_069487-MU
Other Names:
R9694 (G1)
Major Collection:

Strain Information

Npc2
Name: NPC intracellular cholesterol transporter 2
Synonyms: HE1, 2700012J19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67963
VEGA: 12
Homologene: 4697
Slc12a6
Name: solute carrier family 12, member 6
Synonyms: KCC3, gaxp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107723
Homologene: 21069
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7, alpha7 nicotinic receptor, alpha7-nAChR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11441
Homologene: 593
Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 87,153,131 bp (GRCm38)
  • A to G, chromosome 1 at 91,376,251 bp (GRCm38)
  • A to T, chromosome 1 at 93,022,451 bp (GRCm38)
  • A to T, chromosome 1 at 166,101,085 bp (GRCm38)
  • T to C, chromosome 1 at 171,253,961 bp (GRCm38)
  • T to C, chromosome 1 at 178,916,250 bp (GRCm38)
  • T to C, chromosome 2 at 59,795,852 bp (GRCm38)
  • T to C, chromosome 2 at 75,977,347 bp (GRCm38)
  • T to C, chromosome 2 at 86,160,337 bp (GRCm38)
  • T to C, chromosome 2 at 86,298,374 bp (GRCm38)
  • T to A, chromosome 2 at 112,344,536 bp (GRCm38)
  • T to A, chromosome 2 at 143,990,179 bp (GRCm38)
  • A to G, chromosome 3 at 36,018,943 bp (GRCm38)
  • C to T, chromosome 3 at 54,715,594 bp (GRCm38)
  • CAGCACGGTGCGTGAGC to CAGC, chromosome 3 at 88,482,550 bp (GRCm38)
  • T to A, chromosome 3 at 93,826,849 bp (GRCm38)
  • A to T, chromosome 4 at 134,279,400 bp (GRCm38)
  • G to A, chromosome 4 at 139,829,508 bp (GRCm38)
  • T to A, chromosome 5 at 8,849,573 bp (GRCm38)
  • A to G, chromosome 5 at 24,477,141 bp (GRCm38)
  • T to A, chromosome 5 at 61,809,117 bp (GRCm38)
  • A to T, chromosome 5 at 96,781,686 bp (GRCm38)
  • A to G, chromosome 5 at 122,459,645 bp (GRCm38)
  • A to T, chromosome 5 at 135,244,240 bp (GRCm38)
  • A to G, chromosome 5 at 142,487,623 bp (GRCm38)
  • A to T, chromosome 5 at 145,986,875 bp (GRCm38)
  • A to T, chromosome 6 at 22,959,695 bp (GRCm38)
  • T to C, chromosome 6 at 54,489,019 bp (GRCm38)
  • T to A, chromosome 6 at 100,733,213 bp (GRCm38)
  • G to A, chromosome 7 at 18,263,102 bp (GRCm38)
  • A to G, chromosome 7 at 19,580,302 bp (GRCm38)
  • A to T, chromosome 7 at 47,335,520 bp (GRCm38)
  • A to G, chromosome 7 at 63,105,061 bp (GRCm38)
  • A to C, chromosome 7 at 80,325,655 bp (GRCm38)
  • A to T, chromosome 7 at 102,670,804 bp (GRCm38)
  • T to A, chromosome 8 at 71,914,071 bp (GRCm38)
  • T to C, chromosome 9 at 66,919,542 bp (GRCm38)
  • C to T, chromosome 9 at 71,725,521 bp (GRCm38)
  • A to G, chromosome 9 at 100,928,098 bp (GRCm38)
  • A to T, chromosome 9 at 107,744,953 bp (GRCm38)
  • T to C, chromosome 9 at 109,746,067 bp (GRCm38)
  • A to T, chromosome 10 at 76,225,379 bp (GRCm38)
  • T to A, chromosome 10 at 80,333,559 bp (GRCm38)
  • C to T, chromosome 11 at 3,220,384 bp (GRCm38)
  • T to C, chromosome 11 at 7,144,774 bp (GRCm38)
  • T to C, chromosome 11 at 34,268,054 bp (GRCm38)
  • A to G, chromosome 11 at 50,668,145 bp (GRCm38)
  • A to C, chromosome 11 at 83,423,344 bp (GRCm38)
  • T to C, chromosome 11 at 109,463,496 bp (GRCm38)
  • T to C, chromosome 11 at 116,447,120 bp (GRCm38)
  • T to C, chromosome 11 at 121,216,987 bp (GRCm38)
  • A to T, chromosome 12 at 28,535,554 bp (GRCm38)
  • C to A, chromosome 12 at 84,760,864 bp (GRCm38)
  • T to A, chromosome 12 at 112,735,559 bp (GRCm38)
  • G to A, chromosome 13 at 22,187,949 bp (GRCm38)
  • A to T, chromosome 13 at 25,102,357 bp (GRCm38)
  • A to T, chromosome 13 at 95,701,542 bp (GRCm38)
  • C to T, chromosome 14 at 31,154,944 bp (GRCm38)
  • A to G, chromosome 14 at 50,515,212 bp (GRCm38)
  • A to G, chromosome 14 at 52,203,884 bp (GRCm38)
  • C to T, chromosome 14 at 52,314,667 bp (GRCm38)
  • T to C, chromosome 14 at 70,615,292 bp (GRCm38)
  • A to G, chromosome 14 at 121,581,379 bp (GRCm38)
  • A to G, chromosome 15 at 41,102,314 bp (GRCm38)
  • A to T, chromosome 15 at 76,723,728 bp (GRCm38)
  • A to T, chromosome 16 at 32,843,111 bp (GRCm38)
  • C to T, chromosome 17 at 25,817,839 bp (GRCm38)
  • T to G, chromosome 17 at 26,624,611 bp (GRCm38)
  • C to T, chromosome 17 at 27,115,953 bp (GRCm38)
  • A to G, chromosome 17 at 66,809,489 bp (GRCm38)
  • T to C, chromosome 18 at 12,819,508 bp (GRCm38)
  • T to A, chromosome 18 at 36,744,787 bp (GRCm38)
  • A to T, chromosome 18 at 46,900,542 bp (GRCm38)
  • G to A, chromosome 19 at 4,288,483 bp (GRCm38)
  • T to C, chromosome 19 at 4,750,507 bp (GRCm38)
  • A to T, chromosome 19 at 12,901,657 bp (GRCm38)
  • T to G, chromosome 19 at 30,786,971 bp (GRCm38)
  • T to G, chromosome 19 at 34,234,135 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9694 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069487-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.