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Strain Name:
C57BL/6J-MtgxR9695Btlr/Mmmh
Stock Number:
069488-MU
Citation ID:
RRID:MMRRC_069488-MU
Other Names:
R9695 (G1)
Major Collection:

Strain Information

Otx2
Name: orthodenticle homeobox 2
Synonyms: E130306E05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18424
HGNC: HGNC:8522
Homologene: 11026
Neurog2
Name: neurogenin 2
Synonyms: Math4A, ngn2, Atoh4, bHLHa8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11924
Homologene: 7633
Il4i1
Name: interleukin 4 induced 1
Synonyms: Fig1-ps, Fig1, H46, H4, H-46, H-4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14204
Homologene: 22567
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Dvl2
Name: dishevelled segment polarity protein 2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13543
HGNC: HGNC:3086
Homologene: 20927
Heatr1
Name: HEAT repeat containing 1
Synonyms: B130016L12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 217995
VEGA: 13
Homologene: 34562
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to C, chromosome 1 at 16,665,435 bp (GRCm38)
  • A to G, chromosome 1 at 92,588,873 bp (GRCm38)
  • T to C, chromosome 1 at 173,473,683 bp (GRCm38)
  • G to T, chromosome 2 at 28,560,949 bp (GRCm38)
  • A to T, chromosome 2 at 82,975,882 bp (GRCm38)
  • T to C, chromosome 2 at 86,508,756 bp (GRCm38)
  • T to A, chromosome 2 at 90,708,142 bp (GRCm38)
  • T to C, chromosome 2 at 91,162,584 bp (GRCm38)
  • C to T, chromosome 2 at 127,640,388 bp (GRCm38)
  • C to G, chromosome 2 at 172,037,549 bp (GRCm38)
  • T to A, chromosome 2 at 174,425,130 bp (GRCm38)
  • A to G, chromosome 3 at 55,126,534 bp (GRCm38)
  • T to A, chromosome 3 at 82,376,985 bp (GRCm38)
  • T to A, chromosome 3 at 127,634,045 bp (GRCm38)
  • T to A, chromosome 3 at 154,250,951 bp (GRCm38)
  • T to C, chromosome 4 at 115,024,181 bp (GRCm38)
  • T to C, chromosome 4 at 127,122,547 bp (GRCm38)
  • T to A, chromosome 4 at 137,538,390 bp (GRCm38)
  • G to A, chromosome 5 at 114,935,995 bp (GRCm38)
  • T to C, chromosome 5 at 120,197,921 bp (GRCm38)
  • A to T, chromosome 5 at 124,001,853 bp (GRCm38)
  • G to A, chromosome 5 at 127,617,336 bp (GRCm38)
  • T to G, chromosome 6 at 32,206,121 bp (GRCm38)
  • A to T, chromosome 6 at 43,167,576 bp (GRCm38)
  • A to G, chromosome 6 at 108,401,350 bp (GRCm38)
  • G to T, chromosome 6 at 113,047,661 bp (GRCm38)
  • A to T, chromosome 7 at 44,839,609 bp (GRCm38)
  • A to G, chromosome 7 at 90,427,344 bp (GRCm38)
  • G to A, chromosome 8 at 83,938,431 bp (GRCm38)
  • G to T, chromosome 8 at 95,370,786 bp (GRCm38)
  • A to G, chromosome 9 at 19,137,875 bp (GRCm38)
  • A to G, chromosome 9 at 86,755,708 bp (GRCm38)
  • A to G, chromosome 9 at 103,084,940 bp (GRCm38)
  • G to A, chromosome 9 at 108,086,284 bp (GRCm38)
  • A to T, chromosome 9 at 114,386,310 bp (GRCm38)
  • T to C, chromosome 9 at 122,166,574 bp (GRCm38)
  • A to G, chromosome 10 at 5,318,461 bp (GRCm38)
  • A to G, chromosome 10 at 13,474,164 bp (GRCm38)
  • A to T, chromosome 10 at 75,821,238 bp (GRCm38)
  • A to G, chromosome 10 at 81,523,918 bp (GRCm38)
  • C to T, chromosome 10 at 122,448,940 bp (GRCm38)
  • T to C, chromosome 10 at 130,362,416 bp (GRCm38)
  • G to A, chromosome 11 at 70,009,150 bp (GRCm38)
  • A to T, chromosome 11 at 95,055,694 bp (GRCm38)
  • T to C, chromosome 12 at 115,623,367 bp (GRCm38)
  • T to C, chromosome 13 at 12,423,743 bp (GRCm38)
  • A to G, chromosome 13 at 38,058,700 bp (GRCm38)
  • G to A, chromosome 13 at 67,139,134 bp (GRCm38)
  • C to A, chromosome 13 at 108,279,708 bp (GRCm38)
  • T to A, chromosome 14 at 26,462,341 bp (GRCm38)
  • T to G, chromosome 14 at 48,662,495 bp (GRCm38)
  • A to G, chromosome 14 at 70,389,900 bp (GRCm38)
  • T to G, chromosome 15 at 6,786,529 bp (GRCm38)
  • A to T, chromosome 16 at 20,604,225 bp (GRCm38)
  • T to C, chromosome 16 at 45,547,663 bp (GRCm38)
  • T to A, chromosome 16 at 46,099,047 bp (GRCm38)
  • A to G, chromosome 17 at 21,025,484 bp (GRCm38)
  • T to A, chromosome 17 at 28,531,019 bp (GRCm38)
  • A to G, chromosome 17 at 35,012,588 bp (GRCm38)
  • A to G, chromosome 18 at 20,399,332 bp (GRCm38)
  • A to T, chromosome 18 at 43,438,127 bp (GRCm38)
  • A to G, chromosome 18 at 57,477,113 bp (GRCm38)
  • A to G, chromosome 18 at 64,467,732 bp (GRCm38)
  • G to A, chromosome 19 at 3,902,360 bp (GRCm38)
  • T to C, chromosome 19 at 11,651,630 bp (GRCm38)
  • G to T, chromosome 19 at 44,112,986 bp (GRCm38)
  • T to C, chromosome 19 at 57,182,307 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9695 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069488-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.