Strain Name:
C57BL/6J-Ambnem3Odk/Mmnc
Stock Number:
069498-UNC
Citation ID:
RRID:MMRRC_069498-UNC
Other Names:
AMBN-CreERT2

Strain Information

Ambnem3Odk
Name: ameloblastin; endonuclease-mediated mutation 3, Ophir Klein
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Ambn
Name: ameloblastin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11698
HGNC: HGNC:452
Homologene: 7625
Genetic Alterations
CRISPR/Cas9 induced deletion of ORF base positions 4-17 and two base pairs downstream of ORF base position 17 in intron 1, and insertion of a Cre recombinase, linker, ERT2 sequence, and a SV40 polyadenylation sequence after ORF base position 3 of the Ambn gene on the 5th chromosome. This alteration is predicted to induce the expression of Cre recombinase instead of the Ambn protein in cells where and when the Ambn gene is normally expressed upon induction with Tamoxifen.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Hom:The phenotype of this homozygote has not been investigated.Het/Hemi: Heterozyous mice of this phenotype express Cre recombinase in secretory stage ameloblasts upon induction with Tamoxifen.
    Strain Development
    Single-stranded template DNA and guide RNAs were directly microinjected into C57B6/J embryos and implanted into foster mothers to result in gene-edited F0 mice. F0 mice with the desired mutation were mated with homozygous B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (MGI:3809524) mice to produce F1 mice, which were mated with each other to establish a colony and characterize the biological expression of the Cre recombinase. The tdTomato allele was bred out by mating with C57B6/J mice and males were cryopreserved as sperm, available for depositing into the repository.
    Suggested Control Mice
    C57BL/6J mice
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Ophir Klein, M.D., University of California, San Francisco.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    MMRRC Breeding System
    Backcross
    Generation
    N2
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined N/A
    Homozygotes are fertile: Undetermined Undetermined N/A
    Heterozygotes are fertile: Yes Yes N/A
    Age Reproductive Decline: Undetermined Undetermined
    Bred to Homozygosity
    No
    Average litter size
    4 to 6
    Recommended wean age
    3 weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    069498-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.