Strain Detail Sheet

Strain Name:
C57BL/6J-Klk4em4Odk/Mmucd
Stock Number:
069501-UCD
Citation ID:
RRID:MMRRC_069501-UCD
Other Names:
KLK4-MCHERRY

Strain Information

Gene Details

Klk4em4Odk
Name: kallikrein related-peptidase 4 (prostase, enamel matrix, prostate); endonuclease-mediated mutation 4, Ophir D Klein
Synonyms: KLK4-MCHERRY
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Klk4
Name: kallikrein related-peptidase 4 (prostase, enamel matrix, prostate)
Synonyms: Prss17, KLK-L1, ESMP1
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 56640
HGNC: HGNC:6365
Homologene: 55856
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Genetic Alterations
CRISPR/Cas9 induced deletion of ORF base positions 4-64 and two base pairs downstream of ORF base position 64 in intron 2, and insertion of a gene for mcherry fluorescent protein, and a SV40 polyadenylation sequence after ORF base position 3 of the Klk4 gene on the 9th chromosome. This alteration is predicted to induce the expression of mcherry fluorescent protein instead of the Klk4 protein in cells where and when the Klk4 gene is normally expressed.

Strain Description

Phenotype
Hom:The phenotype of this homozygote has not been investigated.Het/Hemi: Heterozygous KLK4-mcherry mice express mcherry in maturation stage ameloblasts.
Strain Development
Single-stranded template DNA and guide RNAs were directly microinjected into C57B6/J embryos and implanted into foster mothers to result in gene-edited F0 mice. F0 mice with the desired mutation were mated with C57B6/J mice to produce F1 mice, which were mated again with C57B6/J mice to establish a colony. Males were cryopreserved as sperm, available for depositing into the repository.
Suggested Control Mice
C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Strain Origin

Donor
Ophir Klein, M.D., University of California, San Francisco.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N2
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined N/A
Homozygotes are fertile: Undetermined Undetermined N/A
Heterozygotes are fertile: Yes Yes N/A
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
4 to 6
Recommended wean age
3 weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.