Strain Detail Sheet
This model harbors a nucleic acid alteration predicting a p.Ser82Met substitution in exon 3 of the Ace2 (angiotensin I converting enzyme (peptidyl-dipeptidase A) 2) gene on the X chromosome. The alteration is designed to partially humanize the mouse Ace2 coding sequence in a region important for binding to the SARS-CoV-2 spike protein.
- Genbank RefSeq - mRNA: NM_001130513.1
- Genbank RefSeq, protein: NP_001123985.1
- Variant, nucleic acid level: c.245_246delinsTG
- Variant, amino acid level, predicted: p.Ser82Met (S82M)
- Check this variant: LUMC Mutalyzer
CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a nucleic acid alteration predicting an amino acid substitution at position 82 altering serine to methionine (S82M - AGT to ATG) in exon 3 of the Ace2 (angiotensin I converting enzyme (peptidyl-dipeptidase A) 2) gene on the X chromosome. Single guide RNA and Cas9 endonuclease were introduced into C57BL/6J-derived fertilized eggs by pronuclear injection. Embryos were transferred to pseudopregnant females, and correctly targeted pups were bred to C57BL/6J mice for germline transmission. The sequence was confirmed by Sanger sequencing; off-target screening has not been performed. The colony was maintained on the C57BL/6J background.
- Immunology and Inflammation
- Models for Human Disease
- Virology
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | X-linked | |
| Homozygotes are fertile: | Yes | X-linked | |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 069508-JAX-SPERM | Cryo-preserved spermatozoa | $459.00 / Non-Profit | Aliquot | Approximate quantity3 |
| 069508-JAX-RESUS | Litter recovered from cryo-archive | $2,123.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.