Strain Name:
STOCK Bard1tm4.2Rjb/Mmmh
Stock Number:
069511-MU
Citation ID:
RRID:MMRRC_069511-MU
Other Names:
Bard1co-rec

Strain Information

Bard1tm4.2Rjbr
Name: BRCA1 associated RING domain 1; targeted mutation 4.2, Richard J Baer
Synonyms: Bard1co-rec
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Bard1
Name: BRCA1 associated RING domain 1
Synonyms: ENSMUSG00000073653, ENSMUSG00000060893
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 12021
HGNC: HGNC:952
Homologene: 400
Genetic Alterations
The Bard1co-rec allele lacks the first coding exon of Bard1. The neomycin selection cassette was removed.
ES Cell Line
KV1 derived from (C57BL/6J x 129S6/SvEvTac)F1
Phenotype
While heterozygous Bard1co-rec/+ mice develop normally and are fertile, homozygous Bard1co-rec/co-rec mice (Null) undergo embryonic lethality prior to gastrulation.
MeSH Terms
  • Animals
  • BRCA1 Protein
  • Chromosomal Instability/genetics
  • DNA Breaks, Double-Stranded
  • DNA Repair/genetics
  • Female
  • Humans
  • Mice
  • Mutation
  • Protein Domains/genetics
  • Recombinational DNA Repair/genetics
  • Tumor Suppressor Proteins/genetics
  • Ubiquitin-Protein Ligases/genetics
Strain Development
To generate a conditional-null Bard1co allele in which the first coding exon is flanked by loxP recombination signals, KV1 ES cells were electroporated with a Bard1co-neo targeting construct. Within this targeting construct, a single loxP signal was inserted ~900 base pairs upstream of the first Bard1 coding exon, while a second loxP signal was inserted, along with a PGK-neomycin resistance cassette flanked by FRT signals ~350 base pairs downstream of the first coding exon. Neomycin-resistant ES clones were injected into blastocysts to obtain germline-transformed mouse strains bearing the Bard1co-neo allele. To excise the FRT-PGK-neo-FRT cassette, chimeric male Bard1co-neo/+ mice were mated with Flpe-expressing (B6.Cg-Tg(ACTFLPe)9205Dym/J, RRID:IMSR_JAX:005703) females. Heterozygous Bard1co/+ mice were then backcrossed with pure C57BL/6J mice twice. The Bard1co-rec allele was then generated by mating with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (RRID:IMSR_JAX:003724). The Bard1co-rec/+ mice were intercrossed to obtain homozygotes.
Suggested Control Mice
Bard1co mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Richard Baer, Ph.D., Columbia University Medical Center.
Primary Reference

Billing D, Horiguchi M, Wu-Baer F, Taglialatela A, Leuzzi G, Nanez SA, Jiang W, Zha S, Szabolcs M, Lin CS, Ciccia A, Baer R. The BRCT Domains of the BRCA1 and BARD1 Tumor Suppressors Differentially Regulate Homology-Directed Repair and Stalled Fork Protection. Mol Cell. 2018 Oct 4;72(1):127-139.e8. doi: 10.1016/j.molcel.2018.08.016. Epub 2018 Sep 20. (Medline PMID: 30244837)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N4 (C57BL/6J and two B6-congenic allele modifiers), F?
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Joan Jose Martinez.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Joan Jose Martinez

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069511-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
069511-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.