Strain Name:
B6J.Cg-Lrig1tm2.1(icre/ERT2)Mrc/Mmucd
Stock Number:
069516-UCD
Citation ID:
RRID:MMRRC_069516-UCD
Other Names:
Lrig1(T2A-iCreERT2)

Strain Information

Lrig1tm2.1(icre/ERT2)Mrc
Name: leucine-rich repeats and immunoglobulin-like domains 1; targeted mutation 2.1, Mario R Capecchi
Synonyms: Lrig1T2A-iCreERT2
Type: Allele
Species: Multi-species
Chromosome: 6
Alteration at locus: Knock-In
Lrig1
Name: leucine-rich repeats and immunoglobulin-like domains 1
Synonyms: LIG-1, Img
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 16206
Homologene: 7380
icre/ERT2
Name: tamoxifen-inducible cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Alteration at locus: Knock-In
Genetic Alterations

The targeting vector constructed to generate this allele is available at Addgene. During the plasmid deposit process, Addgene sequenced the entire plasmid with an NGS technology. The T2A-sfGFP-iCre-ERT2-FRT-Neo*-FRT cassette was knocked in after the coding sequence and before the 3' untranslated region, replacing the endogenous stop codon. The neomycin selection cassette was removed.

ES Cell Line
G4 derived from (129S6/SvEvTac x C57BL/6Ncr)F1
Phenotype
None/Normal/Wild-type
Strain Development
A targeting vector constructed by recombineering from RPCI-24 C57BL/6J mouse genomic DNA library was electroporated into G4 ES cells derived from (129S6/SvEvTac x C57BL/6JNcr)F1. Recombinant clones were injected into C57BL/6J blastocysts. Chimeras were bred to C57BL/6J females. Litters from germline transmitting chimeras were bred to C57BL/6J congenic Tg(Pgk1-FLPo)/+ mice to loop out the Neo cassette. After another generation of crossing to the C57BL/6J mice, Neo-negative mice were further backcrossed to the C57BL/6J mice for a total of 12 generations while fixing the X and Y chromosomes to C57BL/6J (by breeding males or females, etc). Additional information on the development of this strain is available in Figshare.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
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Donor
Mario R. Capecchi, Ph.D., University of Utah.
Primary Reference

Nam HS, Capecchi MR. Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. (Medline PMCID: 7077007)

Colony and Husbandry Information

Another Lrig1 creERT2 knock-in knock-out allele is available at The Jackson Laboratory (STOCK Lrig1tm1.1(cre/ERT2)Rjc/J, RRID:IMSR_JAX:018418). As noted in the corresponding strain information, homozygous females of that line do not lactate well and do not reliably wean newborn pups. The line described here, conversely, does not disrupt Lrig1 expression. The donor bred this line as homozygotes without any problems. The allele here might be useful when generating large numbers of experimental mice: it reduces the cost involved because genotyping isn't necessary for the experimental mice.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N12 (C57BL/6J), F?
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Eric Paulsen.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Eric Paulsen

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069516-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $7,533.58
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.