Strain Detail Sheet

Strain Name:
STOCK Tg(Pou5f1*-rtTA)4Cpl/Mmjax
Stock Number:
069517-JAX
Citation ID:
RRID:MMRRC_069517-JAX
Other Names:
Oct4-rtTA.4, Oct4-rtTA.M2.4

Strain Information

Gene Details

Tg(Pou5f1*-rtTA)4Cpl
Name: transgene insertion 4, Blanche Capel
Type: Allele
Species: Multi-species
Chromosome: Unknown
rtTA
Name: reverse tetracycline transactivator
Type: Gene
Species: Multi-species
Chromosome:
Pou5f1
Name: POU domain, class 5, transcription factor 1
Synonyms: Otf4, Otf3-rs7, Otf-4, Otf-3, Oct-4, Oct-3, Oct-3/4, Oct3/4, Oct4, Otf3g, Otf3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18999
Homologene: 8422
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Genetic Alterations
A BAC clone (RP21-11805) containing the mouse Pou5f1 gene was modified via standard BAC recombineering to contain rtTA-advanced (aka rtTA.M2, hereafter simply referred to as rtTA) led by a KOZAK translation initiation sequence, and a bGHpA polyadenylation sequence at the initiating ATG codon of the first coding exon of Pou5f1.
Genotype Determination

Strain Description

Phenotype

rtTA-advanced (aka rtTA.M2) under the control of the Pou5f1 (Oct4) promoter in inner cell mass of blastocyst, endoderm of epiblast, and E7.5 embryo and extra-embryonic tissue. The allele also causes the development of a "kinky tail" visible as early as E13.5, which is of interest in spine research.

Strain Development
The BAC transgene was linearized and injected into the pronucleus of fertilized F2 mouse embryos from the B6SJLF1/J strain (RRID:IMSR_JAX:100012) using standard microinjection methods. Biopsies were collected from progeny before weaning to identify founders by PCR. Subsequent outcross to CD1. Import to the MMRRC used C57BL/6J.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Developmental Biology
  • Reproduction
  • Research Tools

Strain Origin

Donor
Blanche Capel, Ph.D., Duke University Medical Center.
Primary Reference
In preparation, submitted, or in press

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Agouti
Eye
Black

Breeding

MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: N/A N/A
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.