Strain Name:
C57BL/6J-Igs2em1(SCNN1D*)Hoji/Mmucd
Stock Number:
069550-UCD
Citation ID:
RRID:MMRRC_069550-UCD
Other Names:
SCNN1D knock-in

Strain Information

Igs2tm1(SCNN1D*)Hoji
Name: intergenic region 2; targeted mutation 1, Ji Honglong
Type: Allele
Species: Multi-species
Chromosome: 11
Igs2
Name: intergenic site 2
Synonyms: Hipp11, Iis2, H11
Type: DNA Segment
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: CRISPR
SCNN1D
Name: sodium channel epithelial 1 subunit delta
Type: Gene
Species: Homo sapiens (human)
Chromosome: 1
Alteration at locus: CRISPR
Genetic Alterations
Human SCNN1D knocked into the mouse Hipp11 (Igs2) region.
Genotype Determination

Phenotype
Unknown
MeSH Terms
  • Alveolar Epithelial Cells/metabolism
  • Animals
  • Epithelial Sodium Channels/genetics
  • Epithelial Sodium Channels/metabolism
  • Gene Expression
  • Humans
  • Ion Transport/genetics
  • Ion Transport/physiology
  • Mice
  • Mice, Transgenic/metabolism
  • Models, Animal
  • Oocytes
  • Stem Cells/metabolism
  • Xenopus
Strain Development
CRISPR/Cas9 constructs were microinjected into C57BL/6J zygotes, and the progeny were screened for the desired mutation. Founders were mated to C57BL/6J; the resulting pups (N1) were genotyped via PCR. Additional backcrossing to C57BL/6J (>N5).
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Honglong (James) Ji, M.D., The University of Texas at Tyler Health Science Center.
Primary Reference

Zhao R, Ali G, Chang J, Komatsu S, Tsukasaki Y, Nie HG, Chang Y, Zhang M, Liu Y, Jain K, Jung BG, Samten B, Jiang D, Liang J, Ikebe M, Matthay MA, Ji HL. Proliferative regulation of alveolar epithelial type 2 progenitor cells by human Scnn1d gene. Theranostics. 2019 Oct 18;9(26):8155-8170. doi: 10.7150/thno.37023. (Medline PMID: 31754387)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N5+ (C57BL/6J), F?
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069550-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.