Strain Name:
B6J.129P2-Pofut2Gt(RST434)Byg/BchMmjax
Stock Number:
069554-JAX
Citation ID:
RRID:MMRRC_069554-JAX
Other Names:
Pofut2 RST/+

Gene Information

Pofut2Gt(RST434)Byg
Name: protein O-fucosyltransferase 2; gene trap RST434, BayGenomics
Synonyms: RST434
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Gene Trap
Pofut2
Name: protein O-fucosyltransferase 2
Synonyms: FUT13, 2310011G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Gene Trap
NCBI: 80294
VEGA: 10
Homologene: 12724
Genetic Alterations
High-throughput gene trapping was performed by inserting a gene trap vector containing a splice-acceptor sequence upstream of a reporter gene, β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II), into an intronic or coding region of genomic DNA. The resulting insertional mutation creates a fusion transcript containing sequences from exons upstream of the insertion joined to the β-geo marker, allowing cell lines where the vector has successfully interrupted a gene to be identified.
Genotype Determination
ES Cell Line
E14TG2a.4
Phenotype

These mice were produced from MMRRC:006225-UCD: BayGenomics ES cell line RST434. Homozygotes are embryonic lethal at about E8.5. Heterozygotes are normal, viable, and fertile. Additionally, the strain was backcrossed to C57BL/6J for 31 generations.

Mammalian Phenotype Terms
Allelic Composition: Pofut2Gt(RST434)Byg/Pofut2Gt(RST434)Byg (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Pofut2Gt(OST231463)Lex/Pofut2Gt(RST434)Byg (Genetic Background: involves: 129P2/OlaHsd * 129S5/SvEvBrd )

MeSH Terms
  • Animals
  • Cell Differentiation
  • Endothelial Cells/cytology
  • Epithelial Cells/cytology
  • Extracellular Matrix Proteins/metabolism
  • Female
  • Fucose/metabolism
  • Fucosyltransferases/physiology
  • Gastrulation
  • Germ Layers/cytology
  • Humans
  • Male
  • Mesoderm/cytology
  • Mice
  • Pregnancy
  • Protein Processing, Post-Translational
  • Repressor Proteins/physiology
  • Signal Transduction
  • Thrombospondins/metabolism
  • Transforming Growth Factor beta/physiology
  • ADAMTS9 Protein/genetics
  • ADAMTS9 Protein/metabolism
  • ADAMTS9 Protein/physiology
  • Amnion/embryology
  • Body Patterning
  • Cell Line
  • Embryonic Stem Cells
  • Fucosyltransferases/genetics
  • Gastrulation/genetics
  • HEK293 Cells
  • Mesoderm/embryology
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
Strain Development
BayGenomics ES cell line RST434, MMRRC Stock Number:006225-UCD, were injected into B6 blastocysts at UC Davis. Chimeric mice were shipped to Stony Brook University and have been backcrossed to C57BL/6J for 31 generations.
Suggested Control Mice
C57BL/6J
  • Cell Biology
  • Developmental Biology
Donor
Bernadette Holdener, Stony Brook University.
Primary Reference

Du J, Takeuchi H, Leonhard-Melief C, Shroyer KR, Dlugosz M, Haltiwanger RS, Holdener BC. O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation. Dev Biol. 2010 Oct 1;346(1):25-38. doi: 10.1016/j.ydbio.2010.07.008. Epub 2010 Jul 14. (Medline PMID: 20637190)

Benz BA, Nandadasa S, Takeuchi M, Grady RC, Takeuchi H, LoPilato RK, Kakuda S, Somerville RPT, Apte SS, Haltiwanger RS, Holdener BC. Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. Dev Biol. 2016 Aug 1;416(1):111-122. doi: 10.1016/j.ydbio.2016.05.038. Epub 2016 Jun 10. (Medline PMID: 27297885)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N31 (C57BL/6J), F?
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Valery Matthys

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069554-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
069554-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.