CRISPR-mediated knock-in using C57BL/6J zygotes for injection of Cas9 protein, guide RNA, and a donor plasmid for homologous recombination. The line was sequence-confirmed and backcrossed to C57BL/6J for 10 generations and subsequently sib-mated.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
The availability level for this product has not been determined.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin