Strain Name:
C57BL/6J-Mtnr1aem1Blr/Mmnc
Stock Number:
069562-UNC
Citation ID:
RRID:MMRRC_069562-UNC
Other Names:
Mtnr1a_EGFP_Ct_IRES_ECE
Major Collection:

Gene Information

Mtnr1aem1Blr
Name: elatonin receptor 1A, endonuclease-mediated mutation 1, Bryan Roth
Type: Allele
Species: Multi-species
Chromosome: 8
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Mtnr1a
Name: melatonin receptor 1A
Synonyms: Mel1a receptor, MelR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17773
HGNC: HGNC:7463
Homologene: 21207
Genetic Alterations
Knock-in targeting the Mtnr1a gene, predicting EGFP insertion into the c-terminal tail of this orphan GPCR, proximal to the last transmembrane domain and distal from its predicted PDZ domain. Additionally, separated by an IRES, Cre is knocked into the 3' UTR.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    C57Bl/6J-693
    Phenotype
    None/Normal/Wild-type
    Strain Development

    CRISPR-mediated knock-in using C57BL/6J zygotes for injection of Cas9 protein, guide RNA, and a donor plasmid for homologous recombination. The line was sequence-confirmed and backcrossed to C57BL/6J for 10 generations and subsequently sib-mated.

    Suggested Control Mice
    C57BL/6J
    • Cell Biology
    • Developmental Biology
    • Internal/Organ
    • Neurobiology
    • Research Tools
    • Sensorineural
    Donor
    Bryan Roth, M.D., UNC Chapel Hill.
    Primary Reference
    Not ready to publish

    Colony and Husbandry Information

    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and sib-mating.
    Generation
    N10 (C57BL/6J), F1+
    Overall Breeding Performance
    Excellent
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    The availability level for this product has not been determined.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.