Strain Name:
129S1.Cg-Rbn2tm1Rmz/RmzMmnc
Stock Number:
069572-UNC
Citation ID:
RRID:MMRRC_069572-UNC
Other Names:
Fbn2 null

Strain Information

Fbn2tm1Rmz
Name: fibrillin 2; targeted mutation 1, Francesco Ramirez
Synonyms: Fbn2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout
Fbn2
Name: fibrillin 2
Synonyms: sy, Sne, Fib-2
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Knockout
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Genetic Alterations
Exon 1 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region contained the 5' untranslated region and sequence encoding the signal peptide and the first 85 residues of the protein.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    Both heterozygous and homozygous mice are viable, fertile, and have no embryonic lethality. Homozygous mutant mice serve as a model of congenital contractural arachnodactyly (CCA), and are born with contractures of carpal, metacarpal, and phalangeal joints in the forelimbs that disappear within the first few days of postnatal life. Homozygous mice also display bilateral syndactyly, and tend to have stiffer large joints of the hindlimbs when compared to wild-type littermates.
    MeSH Terms
    • Alleles
    • Animals
    • Apoptosis
    • Body Patterning/drug effects
    • Body Patterning/genetics
    • Bone Morphogenetic Protein 4
    • Bone Morphogenetic Protein 7
    • Bone Morphogenetic Proteins/deficiency
    • Bone Morphogenetic Proteins/genetics
    • Bone Morphogenetic Proteins/metabolism
    • Bone Morphogenetic Proteins/pharmacology
    • Chromosomes/genetics
    • Drug Implants
    • Extracellular Matrix/metabolism
    • Fibrillin-2
    • Fibrillins
    • Forelimb/embryology
    • Forelimb/pathology
    • Gene Targeting
    • Hindlimb/embryology
    • Hindlimb/pathology
    • Limb Deformities, Congenital/genetics
    • Limb Deformities, Congenital/pathology
    • Mesoderm/cytology
    • Mice
    • Mice, Knockout
    • Microfibrils/metabolism
    • Microfibrils/pathology
    • Microfilament Proteins/deficiency
    • Microfilament Proteins/genetics
    • Microfilament Proteins/metabolism
    • Signal Transduction/genetics
    • Syndactyly/genetics
    • Syndactyly/metabolism
    • Syndactyly/pathology
    • Transforming Growth Factor beta
    Strain Development
    A 1.2-kb region encompassing exon 1, which contains the 5′ untranslated region of the mRNA, coding for the signal peptide, and the first 85 amino acids of the protein. of Fbn2 was replaced by a pGK-neo cassette. The targeting vector was electroporated and G418-resistant ES cell clones (R1 derived from 129X1/SvJ x 129S1/Sv)F1-Kitl+) were selected. Following implantation, three chimeric animals germline transmission by Southern hybridization5. Mice were bred back to 129S1/SvImJ (RRID:IMSR_JAX:002448) for >10 generations.
    Suggested Control Mice
    129S1/SvImJ (RRID:IMSR_JAX:002448)
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Dermatology
    • Internal/Organ
    • Models for Human Disease
    Donor
    Francesco Ramirez, Ph.D., Icahn School of Medicine at Mount Sinai.
    Primary Reference

    Arteaga-Solis E, Gayraud B, Lee SY, Shum L, Sakai L, Ramirez F. Regulation of limb patterning by extracellular microfibrils. J Cell Biol. 2001 Jul 23;154(2):275-81. doi: 10.1083/jcb.200105046. (Medline PMID: 11470817)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Agouti
    Eye
    Black
    MMRRC Breeding System
    Backcross
    Generation
    N10+ (129S1/SvImJ - RRID:IMSR_JAX:002448)), F0
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    069572-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.