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Availability & Fees Order this Strain
Randomly inserted TCR alpha transgene construct that combines rearranged genomic DNA of Trav14-1*02 and Traj42*01. The promoter driving the expression is described in PMID:3260351. To uniquely identify the associated DNA, please consult the TRAV and TRAJ pages of the International Immunogenetics (IMGT) Information System.
Randomly inserted TCR beta transgene construct that combines rearranged genomic DNA of Trbv2*1, Trbd1, and Trbj2-5. The promoter driving the expression is described in PMID:7714342.To uniquely identify the associated DNA, please consult the TRBV, TRBD, and TRBJ pages of the International Immunogenetics (IMGT) Information System.
This strain was developed using injection into C57BL/6-derived pronuclei. Subsequent backcrossing to A/J mice produced lines that expressed only the alpha chain (MMRRC:69588) or the beta chain transgene (MMRRC:69589), which were crossed to produce this line expressing both alleles. Total backcross generation: N9.
A/J (RRID:IMSR_JAX:000646)
Blüthmann H, Kisielow P, Uematsu Y, Malissen M, Krimpenfort P, Berns A, von Boehmer H, Steinmetz M. T-cell-specific deletion of T-cell receptor transgenes allows functional rearrangement of endogenous alpha- and beta-genes. Nature. 1988 Jul 14;334(6178):156-9. doi: 10.1038/334156a0. (Medline PMID: 3260351)
Kouskoff V, Signorelli K, Benoist C, Mathis D. Cassette vectors directing expression of T cell receptor genes in transgenic mice. J Immunol Methods. 1995 Mar 27;180(2):273-80. doi: 10.1016/0022-1759(95)00002-r. (Medline PMID: 7714342)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.