Strain Detail Sheet

Strain Name:
B6N.129S6-Is(HSA10;19)1Pmv/Mmnc
Stock Number:
069604-UNC
Citation ID:
RRID:MMRRC_069604-UNC
Other Names:
B6NCrl.129S6-BORCS7/AS3MThumanized

Strain Information

Gene Details

Is(HSA10;19)1Pmv
Name: Insertion, human Chr 10, Chr 19, Fernando Pardo-Manuel de Villena 1
Type: Allele
Species: Multi-species
Chromosome: 19
As3mt
Name: arsenite methyltransferase
Synonyms: 2310045H08Rik, Cyt19
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 57344
VEGA: 19
Homologene: 10754
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Borcs7
Name: BLOC-1 related complex subunit 7
Synonyms: 2010012O05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66439
VEGA: 19
Homologene: 16941
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
AS3MT
Name: arsenite methyltransferase
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
BORCS7
Name: BLOC-1 related complex subunit 7
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
Genetic Alterations
Targeted mutation that replaces endogenous genes Borcs7 and As3mt with their human orthologs BORCS7 and AS3MT using homologous recombination.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    129Sv

    Strain Description

    Phenotype
    In this mouse line AS3MT expression closely modeled that of human and differed substantially from expression of the endogeneous As3mt. Detoxification of iAs was less efficient in the humanized mice than in wild-type mice. Profiles for iAs and its methylated metabolites in tissues and excreta of the humanized mice were consistent with those reported in humans. Notably, the humanized mice expressed both the full-length AS3MT that catalyzes iAs methylation and the human-specific AS3MTd2d3 splicing variant that has been linked to schizophrenia. This line also carries a full BORCS7 human gene.
    Strain Development
    A 59,261bo genomic region derived BAC RP11-753C18 (BACPAC Resources Center, Children’s Hospital Oakland Research Institute, Oakland, CA) replace a 60,908bp of mouse chr 19 and carries the entire gene bodies for AS3MT and BORCS7.The parental ES cell line (phnx43) used in these studies was generated as follows: Male and female 129S6/SvEvTac mice (Taconic Bioscience) were mated. Females were checked every morning for copulatory plugs indicative of successful mating. The process of serial expansion of a single inner-cell mass was continued to at least 10 colonies. Further expansion used mechanical disruption and trypsination. This was followed by DMSO cryopreservation in 10% dimethyl sulfoxide (Sigma-Aldrich; Cat. no. D2660), a sample of the cell line was subjected to karyo- type analysis in KaryoLogic Inc. (RTP, NC). The cell line used in this study had a 40, XY (normal male mouse) karyotype.Mouse carriers were backcrossed multiple times to C57BL/6NCrl both males and females. Mice were genotytped in MiniMUGA for several generations and seven homozygous females and two homzygous males were the breeders for the final line ( ). These breeders were all genotyped in MiniMUGA.
    Suggested Control Mice
    C57BL/6NCrl

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Diabetes
    • Metabolism
    • Neurobiology

    Strain Origin

    Donor
    Fernando Pardo-Manuel de Villena, Ph.D., University of North Carolina at Chapel Hill School of Medicine.
    Primary Reference

    Koller BH, Snouwaert JN, Douillet C, Jania LA, El-Masri H, Thomas DJ, Stýblo M. Arsenic Metabolism in Mice Carrying a BORCS7/AS3MT Locus Humanized by Syntenic Replacement. Environ Health Perspect. 2020 Aug;128(8):87003. doi: 10.1289/EHP6943. Epub 2020 Aug 11. (Medline PMCID: 7418654)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    Speed congenics using MiniMUGA. In practical terms, fully congenic (N>10).
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    069604-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.