Strain Name:
129S4/SvJaeSor-Srftm2.1Sor/Mmnc
Stock Number:
069606-UNC
Citation ID:
RRID:MMRRC_069606-UNC
Other Names:
SrfFLAG

Strain Information

Srftm2.1Sor
Name: serum response factor; targeted mutation 2.1, Philippe Sorian
Synonyms: SrfFLAG
Type: Allele
Species: Synthetic
Chromosome: 17
Alteration at locus: Knock-In
Srf
Name: serum response factor
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knock-In
NCBI: 20807
VEGA: 17
Homologene: 31135
Genetic Alterations
The FLAG tag was knocked into the N-terminus of the Srf gene. This strain serves as a control for MMRRC:69605.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    AK7.1 derived from 129S4/SvJaeSor
    Phenotype
    The SrfFLAG strain contains an N-terminal FLAG tag in the gene Srf. The mouse does not exhibit a phenotype.
    MeSH Terms
    • Animals
    • Mice
    • Neural Crest/embryology
    • Serum Response Factor/genetics
    • Serum Response Factor/metabolism
    • Ternary Complex Factors/genetics
    • Ternary Complex Factors/metabolism
    • Transcription Factors/genetics
    • Transcription Factors/metabolism
    Strain Development
    The line was developed with AK7.1 embryonic stem cells derived from 129S4/AvJaeSor. Chimeras were mated to the same background. Cre recombination employing Meox2tm1(cre)Sor allele (MGI:2176174) on 129S4/SvJaeSor removed the selection cassette, after which the animals backcrossed once to 129S4/SvJaeSor and then intercrossed to homozygosity. Sib-mating until archival.
    Suggested Control Mice
    Wild-type
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Research Tools
    Donor
    Philippe Soriano, Ph.D., Icahn School of Medicine at Mount Sinai.
    Primary Reference

    Dinsmore CJ, Soriano P. Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors. Elife. 2022 Jan 19;11:e75106. doi: 10.7554/eLife.75106. (Medline PMID: 35044299)

    Colony and Husbandry Information

    Can be crossed to wild-type and maintained as a heterozygote or maintained as homozygotes with sib-mating.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Agouti
    Eye
    Black
    MMRRC Breeding System
    Sib-mating
    Generation
    N1 (129S4/SvJaeSor), F?
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    069606-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.