Strain Name:
B6;129P2-Lmo3tm2Thr/Mmmh
Stock Number:
069686-MU
Citation ID:
RRID:MMRRC_069686-MU
Other Names:
Lmo3z

Strain Information

Lmo3tm2Thr
Name: LIM domain only 3; targeted mutation 2, Terence H Rabbitts
Synonyms: Lmo3z
Type: Allele
Species: Mus musculus
Chromosome: 6
Alteration at locus: Knockout
Lmo3
Name: LIM domain only 3
Synonyms: Rbtn-3, Rbtn3
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 109593
HGNC: HGNC:6643
Homologene: 38552
Genetic Alterations
Exon 2 of the mouse Lmo3 gene was was disrupted by the insertion of a beta galactosidase reporter. The targeting vector contained an IRES-lacZ gene plus the simian virus 40 polyadenylation site followed by the neomycin cassette. Brain dissection and whole mount stained with X-Gal reveals beta galactosidase expression.
ES Cell Line
E14
Phenotype
Subtle behavioral phenotypes: reduced locomotor activity and decreased anxiety-like behavior.
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Central Nervous System/embryology
  • Central Nervous System/pathology
  • Central Nervous System/physiology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Embryonic and Fetal Development
  • Female
  • Gene Targeting
  • Genotype
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Humans
  • LIM Domain Proteins
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Morphogenesis
  • Mutation
  • Oncogene Proteins/genetics
  • Oncogene Proteins/metabolism
  • Sequence Alignment
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism
  • Alcohol Drinking/genetics
  • Alcohol Drinking/metabolism
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology
  • Brain/drug effects
  • Brain/metabolism
  • Choice Behavior/drug effects
  • Choice Behavior/physiology
  • Ethanol/pharmacology
  • LIM Domain Proteins/genetics
  • LIM Domain Proteins/metabolism
  • Mice, Knockout
  • Anxiety/genetics
  • Anxiety/metabolism
  • Anxiety Disorders/metabolism
  • Basolateral Nuclear Complex/metabolism
  • Corticotropin-Releasing Hormone/metabolism
  • Disease Models, Animal
  • Receptors, Corticotropin-Releasing Hormone/biosynthesis
  • Receptors, Corticotropin-Releasing Hormone/genetics
  • Receptors, Corticotropin-Releasing Hormone/metabolism
Strain Development
The Lmo3 targeting strategy employed the introduction of a BamHI site into Lmo3 exon 2 and cloning the p1056 lacZ reporter. The targeting vector comprised a 6.6-kb BamHI-XhoI fragment cloned in pBSpt. Targeted clones were detected using a 5′ external probe comprising a 1-kb BamHI fragment which was also used to monitor the transmission of the targeted allele in mice. Embryonic stem (ES) cell line used was E14. DNA transfection was carried out by electroporation, and clones were selected by resistance to G418. Targeted clones were confirmed by filter hybridization and injected in donor blastocysts for generation of chimeric mice. Chimeric mice were backcrossed 2 generations to C57BL/6J.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Neurobiology
  • Obesity
Donor
Terence Rabbitts, Ph.D., The Institute of Cancer Research.
Primary Reference

Tse E, Smith AJ, Hunt S, Lavenir I, Forster A, Warren AJ, Grutz G, Foroni L, Carlton MB, Colledge WH, Boehm T, Rabbitts TH. Null mutation of the Lmo4 gene or a combined null mutation of the Lmo1/Lmo3 genes causes perinatal lethality, and Lmo4 controls neural tube development in mice. Mol Cell Biol. 2004 Mar;24(5):2063-73. doi: 10.1128/MCB.24.5.2063-2073.2004. (Medline PMID: 14966285)

Savarese A, Zou ME, Kharazia V, Maiya R, Lasek AW. Increased behavioral responses to ethanol in Lmo3 knockout mice. Genes Brain Behav. 2014 Nov;13(8):777-83. doi: 10.1111/gbb.12176. Epub 2014 Sep 24. (Medline PMID: 25176312)

Savarese A, Lasek AW. Regulation of anxiety-like behavior and Crhr1 expression in the basolateral amygdala by LMO3. Psychoneuroendocrinology. 2018 Jun;92:13-20. doi: 10.1016/j.psyneuen.2018.03.016. Epub 2018 Mar 27. (Medline PMID: 29609111)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
2 generations to C57BL/6J

Edit:
2


Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Hyunjin Kim.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069686-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
069686-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.