Strain Name:
129S6/SvEvTac-Ace2tm2(ACE2)Bhk/Mmnc
Stock Number:
069710-UNC
Citation ID:
RRID:MMRRC_069710-UNC
Other Names:
129-ACE2

Strain Information

Ace2tm2(ACE2)Bhk
Name: angiotensin converting enzyme 2; targeted mutation 2, Beverly H Koller
Synonyms: hACE2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
Ace2
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 70008
Homologene: 41448
ACE2
Name: angiotensin I converting enzyme 2
Type: Gene
Species: Homo sapiens (human)
Chromosome: X
Alteration at locus: Targeted Mutation
Genetic Alterations
This mouse line was modified by homologous recombination at the Ace2 locus. The regulatory region and coding region of the mouse Ace2 gene was removed and replaced by the regulatory and coding regions of the human ACE2 gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Phnx43
    Phenotype
    This mouse line expresses the human ACE2 mRNA in a pattern that mimics that of human ACE2. This humanized pattern of expression reflects the fact that the entire mouse Ace2 locus is removed, including 5' regulatory regions. This deleted segment of mouse DNA is then populated with the syntenic segment of human DNA including the human protomer and any potential intergenic and 3' regulatory regions. Consistent with this, the splicing pattern for the gene mimics that reported for the human gene. Protein expression is verified in various tissues by Western analysis. In some tissues verification of the presence of active ACE2 is determined by enzymatic assay.

    This mouse line carries an Ace2 allele identical to that described in MMRRC:69715 (B6N.129S6/SvEvTac-Ace2tm2(ACE2)Bhk/Mmnc) and MMRRC:69714 (CBy.129S6/SvEvTac-Ace2tm2(ACE2)Bhk/Mmnc). The primary difference is that this strain is on a 129S6/SvEvTac genetic background.
    Strain Development
    The Ace2 displacer constructs were assembled using a standard recombineering approach. The mouse arms of homology were derived from the 129S7/AB2.2 bMQ BAC library clone bMQ306a09 (Source BioScience). The segment of human genomic DNA containing the ACE2 gene was derived from the human tile path BAC RP11-478H11 (BACPAC Resources). The recombination events for generation was carried out in the 129 ES cell line, Phnx43. ES cells used were derived from 129SvEv mice. ES cells carrying the correctly modified locus were used to generate chimeric mice, which were bred to 129S6/SvEv/Tac mice (Taconic Bioscience) to maintain the mutation on this genetic background.
    Suggested Control Mice
    129S6
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    • Models for Human Disease
    • Virology
    Donor
    Beverly Koller, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference
    In preparation, submitted, or in press

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Agouti
    Eye
    Black
    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Excellent
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes X-linked
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    10 to 14
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    10 to 14

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    069710-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.