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Availability & Fees Order this Strain
A transgene construct containing the following elements, in order, randomly inserted into the genome: the Tie2 promoter, a 1.3kb BamHI/NaeI Gja5 (Cx40) DNA segment, an IRES, the EGFP fluorescent reporter gene, a human growth hormone polyadenylation signal, and a Tie2 enhancer. The Tie2 promoter drives expression in the endothelium. LacO sites were introduced into the Tie2 promoter to enable regulation of transgene expression with a LacI repressor protein. A single base mutation encoding the amino acid at position 152 (T152A) was incorporated into the transgenic construct.
The strain exhibits no unusual overt phenotype. The donating investigators observed EGFP expression in several types of endothelium. The presence of LacO sites introduced into the Tie2 promoter enables regulation of transgene expression with a LacI repressor protein.
Chaston DJ, Baillie BK, Grayson TH, Courjaret RJ, Heisler JM, Lau KA, Machaca K, Nicholson BJ, Ashton A, Matthaei KI, Hill CE. Polymorphism in endothelial connexin40 enhances sensitivity to intraluminal pressure and increases arterial stiffness. Arterioscler Thromb Vasc Biol. 2013 May;33(5):962-70. doi: 10.1161/ATVBAHA.112.300957. Epub 2013 Mar 7. (Medline PMID: 23471232)
Morton SK, Chaston DJ, Howitt L, Heisler J, Nicholson BJ, Fairweather S, Bröer S, Ashton AW, Matthaei KI, Hill CE. Loss of functional endothelial connexin40 results in exercise-induced hypertension in mice. Hypertension. 2015 Mar;65(3):662-9. doi: 10.1161/HYPERTENSIONAHA.114.04578. Epub 2014 Dec 29. (Medline PMID: 25547341)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.