Strain Name:
B6.A-Carm1m9Bei/Mmucd
Stock Number:
069760-UCD
Citation ID:
RRID:MMRRC_069760-UCD
Other Names:
Carm1^A296E (MGI:5505489)

Strain Information

Carm1m9Bei
Name: coactivator-associated arginine methyltransferase 1; mutation 9, David R Beier
Synonyms: line 8, Carm1A296E
Type: Allele
Species: Mus musculus
Chromosome: 9
Alteration at locus: Chemically Induced
Carm1
Name: coactivator-associated arginine methyltransferase 1
Synonyms: m9Bei, Prmt4
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 59035
Homologene: 10990
Genetic Alterations
ENU mutagenesis was used to generate this C-to-A mutation that changes alanine codon 296 (GCA) to glutamic acid (GAA) (p.A296E).
ES Cell Line
Not applicable
Phenotype
Carm1 homozygous mutant embryos show a range of developmental abnormalities, including cobblestone-like cortical malformation, cleft palate, craniofacial defects, omphalocele, stiff leg posture, and club foot. Carm1 homozygous mutants show varied cardiac defects. Most notably, many affected mid-gestation embryos appear to have cardiac rupture and hemorrhaging in the thorax. Mice that survive to late gestation display cardiac defects, including ventricular septal defects, double outlet right ventricle, and persistent truncus arteriosus.
MeSH Terms
  • Animals
  • Female
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • PAX3 Transcription Factor
  • Paired Box Transcription Factors/genetics
  • Pregnancy
  • Protein-Arginine N-Methyltransferases
  • X-Ray Microtomography
  • Axons/drug effects
  • Axons/pathology
  • Cell Adhesion Molecules, Neuronal/genetics
  • Cerebral Cortex/abnormalities
  • Cerebral Cortex/drug effects
  • Cerebral Cortex/pathology
  • Ethylnitrosourea/toxicity
  • Extracellular Matrix Proteins/genetics
  • Gene Expression Regulation, Developmental/drug effects
  • Genetic Testing/methods
  • Hydrocephalus/genetics
  • Hydrocephalus/pathology
  • Low Density Lipoprotein Receptor-Related Protein-2/genetics
  • Male
  • Malformations of Cortical Development/genetics
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mutagens/toxicity
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Reelin Protein
  • Serine Endopeptidases/genetics
Strain Development
Eight week old A/J (Jackson Laboratory) male mice were treated weekly for three weeks with ENU. After several weeks of recovery, mice were crossed to C57BL/6 females with or without a lacZ reporter. Resulting females were backcrossed to their fathers and subjected to phenotype screening. The mutation is an C-to-A mutation that changes alanine codon 296 (GCA) to glutamic acid (GAA) (p.A296E).
The mutant line is maintained by serial backcrosses to C57BL/6J.
Suggested Control Mice
B6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Developmental Biology
Donor
David Beier, M.D., Seattle Children's Research Institute.
Primary Reference

Jamet S, Ha S, Ho TH, Houghtaling S, Timms A, Yu K, Paquette A, Maga AM, Greene NDE, Beier DR. The arginine methyltransferase Carm1 is necessary for heart development. G3 (Bethesda). 2022 Jul 29;12(8):jkac155. doi: 10.1093/g3journal/jkac155. (Medline PMID: 35736367)

Ha S, Stottmann RW, Furley AJ, Beier DR. A forward genetic screen in mice identifies mutants with abnormal cortical patterning. Cereb Cortex. 2015 Jan;25(1):167-79. doi: 10.1093/cercor/bht209. Epub 2013 Aug 22. (Medline PMID: 23968836)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Pink
MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069760-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
069760-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.