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Strain Detail Sheet

Strain Name:
STOCK Tg(H2-K-GPX1,-SOD1,-SOD3*)CCpj/Mmmh
Stock Number:
069790-MU
Citation ID:
RRID:MMRRC_069790-MU
Other Names:
TG

Strain Information

Gene Details

Tg(H2-K-GPX1,-SOD1,-SOD3*)CCpj
Name: transgene insertion C, Peter J Cowan
Synonyms: Tg(H2-K-GPX1,-SOD1,-SOD3/CD55)CCpj, SOD1.SOD3.Gpx1.Tg
Type: Allele
Species: Multi-species
Chromosome:
Alteration at locus: Transgenic
Gpx1
Name: glutathione peroxidase 1
Synonyms: CGPx, cellular GPx, GSHPx-1, Gpx, GPx-1
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Transgenic
NCBI: 14775
HGNC: HGNC:4553
Homologene: 20155

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tcra
Name: T cell receptor alpha chain
Synonyms: Tcr alpha, Tcralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Transgenic
Genetic Alterations
Integration of three transgenes that express from the mouse H-2Kb, with a TCRa enhancer driving expression of His-tagged glutathione peroxidase (Gpx1) cDNA.
ES Cell Line
Not applicable

Strain Description

Phenotype

Triple-transgenic islets showed the broadest resistance to oxidative stress in vitro and when grafted provided significantly improved early control of blood glucose compared with double-transgenic, single-transgenic, and wild-type grafts.

Useful to study oxidative stress in diabetes.

MeSH Terms
  • Animals
  • Base Sequence
  • Blood Glucose/metabolism
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary/genetics
  • Diabetes Mellitus, Experimental/blood
  • Glutathione Peroxidase/genetics
  • Isoenzymes/genetics
  • Mice
  • Mice, Transgenic
  • Oxidative Stress/physiology
  • Polymerase Chain Reaction
  • Superoxide Dismutase/genetics
  • Glutathione Peroxidase GPX1
Strain Development
Constructs were prepared by cloning cDNAs for human antioxidant enzymes into a vector containing the mouse H-2Kb promoter. Construction of a modified extracellular SOD cDNA required several steps. First, the extracellular SOD coding region was amplified from human genomic DNA. The downstream primer incorporated a site for the restriction enzyme BspHI to allow the construction of a hybrid extracellular SOD/CD55 cDNA using a previously described strategy (29). The hybrid cDNA encoded an extracellular SOD protein in which the COOH terminus including the heparin-binding domain was replaced by the GPI anchor of human CD55. Finally, a splice-overlap PCR technique (30) was used to incorporate a FLAG epitope immediately downstream of the signal peptide. Constructs were microinjected separately at 10% g/ml into CBA/C57BL6 F1 oocytes. An equimolar mixture at the same total concentration was microinjected to obtain triple-transgenic mice. Transgenic animals were identified by PCR of tail-tip genomic DNA prepared using the QIAamp system.
Suggested Control Mice
TG(-)

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Immunology and Inflammation
  • Metabolism

Strain Origin

Donor
Peter Cowan, Ph.D., University of Melbourne: Melbourne, VIC, AU.
Primary Reference

Mysore TB, Shinkel TA, Collins J, Salvaris EJ, Fisicaro N, Murray-Segal LJ, Johnson LE, Lepore DA, Walters SN, Stokes R, Chandra AP, O'Connell PJ, d'Apice AJ, Cowan PJ. Overexpression of glutathione peroxidase with two isoforms of superoxide dismutase protects mouse islets from oxidative injury and improves islet graft function. Diabetes. 2005 Jul;54(7):2109-16. doi: 10.2337/diabetes.54.7.2109. (Medline PMID: 15983212)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: No Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Research Directorate.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Research Directorate

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
069790-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.