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Availability & Fees Order this Strain
An "EMTB 3XGFP pA Neo" segment was cloned into a BAC containing the Slc1a3 gene (GLAST1). EMTB consists of the microtubule binding domain of Map7 (ensconsin) fused to GFP. Insertion in the Slc1a3 gene occurred at exon 2 (at the ATG). Integration of the transgene by homologous recombination, was followed by selection of the correctly targeted BAC clone, and removal of the neo cassette by Flpe recombination. The BAC was microinjected into fertilized eggs from CBA X C57BL/6 mice.
Conditional phenotype: No
An "EMTB 3XGFP pA Neo" segment was cloned into a BAC containing the Slc1a3 gene (GLAST1). EMTB consists of the microtubule binding domain of Map7 (ensconsin) fused to GFP. Insertion in the Slc1a3 gene occurred at exon 2 (at the ATG). Integration of the transgene by homologous recombination, was followed by selection of the correctly targeted BAC clone, and removal of the neo cassette by Flpe recombination. The BAC was microinjected into fertilized eggs from CBA X C57BL/6 mice. Founder mice were identified and maintained on a C57BL/6 background before being crossed to FVB/N for an unknown number of generations.
Eom TY, Stanco A, Weimer J, Stabingas K, Sibrack E, Gukassyan V, Cheng J, Anton ES. Direct visualization of microtubules using a genetic tool to analyse radial progenitor-astrocyte continuum in brain. Nat Commun. 2011 Aug 23;2:446. doi: 10.1038/ncomms1460. (Medline PMCID: 3905834)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.