Radial progenitor-specific Brainbow mice (RG-Brainbow) differentially label radial glial cells (RGCs ) by expressing mCherry or YFP in a Cre-dependent manner in the embryonic cortex.
Conditional phenotype: Yes. Cre-dependent expression of Brainbow markers in radial progenitors.
The RG-Brainbow mice incorporate a Brainbow1.1M construct under the control of a Slc1a3 (GLAST1) promoter. The Brainbow 1.1 construct has four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5’ to 3’) a LoxN site, Lox2272 site, LoxPsite, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site,mCherry RFP (with membrane tethering palmitoylation sequence and polyAsequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxPsite, mCerulean CFP (with membrane tethering palmitoylation sequence and 5’ FRTsite and polyA sequence). The construct was inserted into a BAC encoding the Slc1a3 gene via homologous recombination (at the ATG site).
The modified BAC was microinjected into fertilized eggs from the CBA X C57BL6 mice. Founders were identified and maintained on a C57BL/6 background for an unknown number of generations.
Nakagawa N, Plestant C, Yabuno-Nakagawa K, Li J, Lee J, Huang CW, Lee A, Krupa O, Adhikari A, Thompson S, Rhynes T, Arevalo V, Stein JL, Molnár Z, Badache A, Anton ES. Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development. Neuron. 2019 Sep 4;103(5):836-852.e5. doi: 10.1016/j.neuron.2019.05.049. Epub 2019 Jul 2. (Medline PMCID: 6728225)
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.Viability and Fertility: | Female | Male | Comments |
---|---|---|---|
Homozygotes are viable: | Yes | Yes | |
Homozygotes are fertile: | Yes | Yes | |
Hetero/Hemizygotes are fertile: | Yes | Yes | |
Age Reproductive Decline: | 7 to 9 months | 7 to 9 months |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
---|---|---|---|---|
069806-UNC-RESUS | Litter recovered from cryo-archive | $3,242.00 / $5,382.00 Non-Profit / For-Profit |
Litter | Recovered litter4; additional fees for any special requests. |
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information. |
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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