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Availability & Fees Order this Strain
A targeting vector was designed to insert loxP sites flanking exon 1 of the Muc5b gene, followed by a frt-flanked neomycin resistance (neo) cassette downstream of exon 1.The construct was electroporated into 129S1-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts, and the resulting chimeras were bred to C57BL/6J mice. Further crosses were performed to Rosa26-FLPe mice and CMV-Cre mice to remove the neo cassette and exon 1, generating the knockout strain.
These knockout mice lack expression of the MUC5B protein which is required for proper mucociliary clearance. Mice exhibit lung inflammation and chronic infection by multiple bacterial species. The donating investigator reports that KO mice may develop mucus plugging in the nasopharynx, which may cause a labored breathing and a hunched appearance. While possible, breeding mice as homozygotes may be difficult due to infection related difficulties.
A targeting vector was designed to insert loxP sites flanking exon 1 of the Muc5b gene, followed by a frt-flanked neomycin resistance (neo) cassette downstream of exon 1.The construct was electroporated into 129S1-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts, and the resulting chimeras were bred to C57BL/6J mice. Further crosses were performed to Rosa26-FLPemice and CMV-Cre mice to remove the neo cassette and exon 1, generating the knockout strain. The line was crossed to C57BL/6J mice for 5 generations before being mated to C57BL/6NTac mice for an additional 12 generations.
Roy MG, Livraghi-Butrico A, Fletcher AA, McElwee MM, Evans SE, Boerner RM, Alexander SN, Bellinghausen LK, Song AS, Petrova YM, Tuvim MJ, Adachi R, Romo I, Bordt AS, Bowden MG, Sisson JH, Woodruff PG, Thornton DJ, Rousseau K, De la Garza MM, Moghaddam SJ, Karmouty-Quintana H, Blackburn MR, Drouin SM, Davis CW, Terrell KA, Grubb BR, O'Neal WK, Flores SC, Cota-Gomez A, Lozupone CA, Donnelly JM, Watson AM, Hennessy CE, Keith RC, Yang IV, Barthel L, Henson PM, Janssen WJ, Schwartz DA, Boucher RC, Dickey BF, Evans CM. Muc5b is required for airway defence. Nature. 2014 Jan 16;505(7483):412-6. doi: 10.1038/nature12807. Epub 2013 Dec 8. (Medline PMCID: 4001806)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.