Strain Name:
B6.Cg-Xpatm1Hvs Ercc8em1(IMPC)H/Mmjax
Stock Number:
069949-JAX
Citation ID:
RRID:MMRRC_069949-JAX
Other Names:
B6.Cg-Xpa^tm1Hvs Ercc8^em1(IMPC)H/Mmjax

Strain Information

Ercc8em1(IMPC)H
Name: excision repaiross-complementing rodent repair deficiency, complementation group 8; endonuclease-mediated mutation 1, Harwell
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Endonuclease-mediated
Ercc8
Name: excision repaiross-complementing rodent repair deficiency, complementation group 8
Synonyms: 2410022P04Rik, 2810431L23Rik, 4631412O06Rik, Csa, B130065P18Rik, Ckn1
Type: Gene
Species: Mouse
Chromosome: 13
Alteration at locus: Endonuclease-mediated
NCBI: 71991
HGNC: HGNC:3439
Homologene: 62
Xpatm1Hvs
Name: xeroderma pigmentosum, complementation group A; targeted mutation 1, Harry van Steeg
Synonyms: XpaKO, XPA-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Xpa
Name: xeroderma pigmentosum, complementation group A
Synonyms: Xpac
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 22590
Homologene: 37298
Genetic Alterations
The XPA- targeting vector used a PGK-neomycin resistance cassette to replace exons 3 and 4 of the Xpa gene on chromosome 4.
ES Cell Line
Unspecified
Phenotype
Mice homozygous for both mutations are predicted to exhibit several CS symptoms including premature death (nearly 100% penetrance by 28), loss of adiposity, and neurological symptoms.
Mammalian Phenotype Terms
Allelic Composition: Xpatm1Hvs/Xpatm1Hvs (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Xpatm1Hvs/Xpatm1Hvs (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Xpatm1Hvs/Xpatm1Hvs (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

Allelic Composition: Xpatm1Gvh/Xpatm1Hvs (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

Strain Development
Mice were recovered from cryopreserved sperm from strains (B6.129P2-Xpa^tm1Hvs/Mmmh Stock No. 016831, and C57BL/6NTac-Ercc8^em1(IMPC)H/Mmnc Stock No. 067231) and then bred together to generate this double mutant.
Suggested Control Mice
C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Cat Lutz, Ph.D., The Jackson Laboratory.
Primary Reference
Not ready to publish

Colony and Husbandry Information

When maintaining a live colony mice are bred as HET (Xpa) HOM (Ercc8) x WT (Xpa) HOM (Ercc8) and the reciprocal mating. Mice homozygous for both mutations are predicted to die by P28 on standard chow, however, introducing soft chow may extend lifespan.
For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
more than 5
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Undetermined Undetermined
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069949-JAX-HET-F
069949-JAX-HET-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.