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Strain Name:
C57BL/6J-MtgxR9723Btlr/Mmmh
Stock Number:
069970-MU
Citation ID:
RRID:MMRRC_069970-MU
Other Names:
R9723 (G1)
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Fasl
Name: Fas ligand
Synonyms: Fas-L, CD178, APT1LG1, CD95L, Tnfsf6, Fasl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14103
Homologene: 533
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Oxtr
Name: oxytocin receptor
Synonyms: OTR
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18430
HGNC: HGNC:8529
Homologene: 20255
Ggta1
Name: glycoprotein galactosyltransferase alpha 1, 3
Synonyms: GALT, alpha Gal, Gal, glycoprotein alpha galactosyl transferase 1, alpha3GalT, Ggta-1, Ggta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14594
HGNC: HGNC:4253
Homologene: 7730
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to C, chromosome 1 at 40,293,561 bp (GRCm38)
  • G to T, chromosome 1 at 71,624,210 bp (GRCm38)
  • C to T, chromosome 1 at 74,364,586 bp (GRCm38)
  • G to A, chromosome 1 at 75,179,722 bp (GRCm38)
  • T to C, chromosome 1 at 131,844,365 bp (GRCm38)
  • T to C, chromosome 1 at 161,787,966 bp (GRCm38)
  • T to G, chromosome 1 at 190,939,145 bp (GRCm38)
  • G to T, chromosome 2 at 35,413,406 bp (GRCm38)
  • A to G, chromosome 2 at 76,771,585 bp (GRCm38)
  • C to T, chromosome 2 at 87,489,675 bp (GRCm38)
  • T to C, chromosome 2 at 111,228,355 bp (GRCm38)
  • A to T, chromosome 2 at 125,217,907 bp (GRCm38)
  • A to T, chromosome 2 at 125,360,199 bp (GRCm38)
  • C to T, chromosome 2 at 126,646,728 bp (GRCm38)
  • A to C, chromosome 2 at 150,472,269 bp (GRCm38)
  • A to G, chromosome 2 at 155,981,417 bp (GRCm38)
  • T to G, chromosome 4 at 41,603,302 bp (GRCm38)
  • C to G, chromosome 4 at 47,119,541 bp (GRCm38)
  • T to G, chromosome 4 at 94,804,302 bp (GRCm38)
  • A to G, chromosome 4 at 99,020,033 bp (GRCm38)
  • A to T, chromosome 4 at 99,072,423 bp (GRCm38)
  • T to A, chromosome 4 at 101,988,886 bp (GRCm38)
  • C to T, chromosome 4 at 116,712,398 bp (GRCm38)
  • A to G, chromosome 4 at 135,425,369 bp (GRCm38)
  • A to G, chromosome 4 at 156,222,418 bp (GRCm38)
  • G to A, chromosome 5 at 20,896,466 bp (GRCm38)
  • G to A, chromosome 5 at 33,390,038 bp (GRCm38)
  • G to T, chromosome 5 at 36,614,940 bp (GRCm38)
  • A to G, chromosome 5 at 52,538,501 bp (GRCm38)
  • A to G, chromosome 5 at 72,160,966 bp (GRCm38)
  • G to A, chromosome 5 at 88,504,382 bp (GRCm38)
  • A to T, chromosome 5 at 90,464,103 bp (GRCm38)
  • A to T, chromosome 5 at 92,066,529 bp (GRCm38)
  • T to A, chromosome 5 at 138,299,841 bp (GRCm38)
  • G to A, chromosome 6 at 112,489,343 bp (GRCm38)
  • C to A, chromosome 6 at 120,793,528 bp (GRCm38)
  • G to A, chromosome 6 at 125,307,385 bp (GRCm38)
  • A to T, chromosome 7 at 23,866,349 bp (GRCm38)
  • C to T, chromosome 7 at 26,210,171 bp (GRCm38)
  • A to T, chromosome 7 at 79,121,988 bp (GRCm38)
  • G to A, chromosome 8 at 3,622,374 bp (GRCm38)
  • A to G, chromosome 8 at 39,589,316 bp (GRCm38)
  • C to T, chromosome 8 at 80,614,723 bp (GRCm38)
  • T to C, chromosome 8 at 104,950,831 bp (GRCm38)
  • G to A, chromosome 9 at 3,385,283 bp (GRCm38)
  • T to A, chromosome 9 at 53,562,446 bp (GRCm38)
  • T to G, chromosome 9 at 53,570,561 bp (GRCm38)
  • A to G, chromosome 9 at 63,146,432 bp (GRCm38)
  • A to G, chromosome 10 at 6,838,514 bp (GRCm38)
  • T to A, chromosome 10 at 13,168,753 bp (GRCm38)
  • A to T, chromosome 10 at 21,996,340 bp (GRCm38)
  • C to T, chromosome 11 at 58,859,622 bp (GRCm38)
  • A to T, chromosome 11 at 82,890,336 bp (GRCm38)
  • A to T, chromosome 11 at 85,044,710 bp (GRCm38)
  • T to A, chromosome 11 at 94,359,899 bp (GRCm38)
  • A to G, chromosome 11 at 97,034,947 bp (GRCm38)
  • A to T, chromosome 11 at 97,334,720 bp (GRCm38)
  • T to C, chromosome 12 at 72,556,265 bp (GRCm38)
  • A to G, chromosome 13 at 23,695,923 bp (GRCm38)
  • T to C, chromosome 13 at 49,590,362 bp (GRCm38)
  • T to C, chromosome 13 at 95,015,163 bp (GRCm38)
  • A to G, chromosome 13 at 96,414,518 bp (GRCm38)
  • G to T, chromosome 14 at 20,725,444 bp (GRCm38)
  • T to A, chromosome 14 at 31,163,891 bp (GRCm38)
  • T to C, chromosome 14 at 31,265,989 bp (GRCm38)
  • T to C, chromosome 14 at 33,955,517 bp (GRCm38)
  • T to C, chromosome 14 at 43,261,684 bp (GRCm38)
  • T to C, chromosome 14 at 60,691,049 bp (GRCm38)
  • T to A, chromosome 15 at 39,828,113 bp (GRCm38)
  • A to G, chromosome 15 at 72,590,114 bp (GRCm38)
  • C to T, chromosome 15 at 102,320,735 bp (GRCm38)
  • T to A, chromosome 16 at 26,624,157 bp (GRCm38)
  • A to T, chromosome 16 at 32,033,832 bp (GRCm38)
  • C to A, chromosome 17 at 24,913,611 bp (GRCm38)
  • A to T, chromosome 17 at 25,750,034 bp (GRCm38)
  • G to A, chromosome 17 at 26,970,536 bp (GRCm38)
  • G to A, chromosome 17 at 56,116,290 bp (GRCm38)
  • A to G, chromosome 17 at 56,318,061 bp (GRCm38)
  • G to T, chromosome 18 at 10,009,993 bp (GRCm38)
  • A to G, chromosome 18 at 12,210,592 bp (GRCm38)
  • A to T, chromosome 19 at 6,839,295 bp (GRCm38)
  • G to C, chromosome 19 at 12,428,933 bp (GRCm38)
  • A to G, chromosome 19 at 34,648,857 bp (GRCm38)
  • T to C, chromosome 19 at 46,134,716 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9723 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069970-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.