Strain Name    : B6.Cg-Myo5aflr Gnb5flr/Mmnc

Stock Number : 000070-UNC

Gene Information

(provided by MGI)
Allele Symbol: Myo5aflr
Name: flailer
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Allele Symbol: Myo5aflr
Name: flailer
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Allele Symbol: Gnb5flr
Name: flailer
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Gene Symbol: Myo5a
Name: myosin VA
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Gene Symbol: Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
Due to unequal recombination within an intron of the two affected genes, the mutant chromosome contains an extra hybrid gene in addition to wildtype copies of Gnb5 and Myo5a. The flailer mice produce a hybrid protein containing the cargo-binding domain of Myosin 5a, which is expressed in neurons only. The hybrid protein acts as a dominant negative inhibitor of transport processes dependent on Myo5a.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line: Not applicable

    Phenotype

    Neurological abnormalities by 14 days postnatal include frequent falling, convulsive limb movements, opisthotonos in response to handling. Unlike the Myo5a-neurological mutants which it resembles at 2-4 weeks of age, flailer mice recover and exhibit only mild ataxia as adults. Adults do not perform well on rotorod. The histological phenotype includes delayed localization of smooth ER in dendritic spines of cerebellar Purkinje cells.

    Mammalian Phenotype Terms:

    Strain of Origin: C57BL/10J, CBA

    Strain genetic background: C57BL/6J

    Strain Development: B6CBACa-AWJ/A, tb2J/tb2J F2 mice were obtained from the frozen embryo repository at the Jackson Laboratory (Bar Harbor, ME). These mice were backcrossed once to strain C57BL/6J and heterozygous flr/+ offspring were intercrossed. flailer has subsequently been maintained by brother–sister mating of homozygous affected animals. The resulting recombinant congenic line, STOCK Myo5aflr Gnb5flr/Unc, was sib mated for at least10 generations. The composition of the background stock was ~75% C57BL/6J and 25% CBA prior to backcrossing to C57BL/6 (N>10).

    Suggested Control Mice:

    • C57BL/6

    • Neurologic. Can be used to study Myo5a dependent processes.

    Donor: Miriam Meisler, Ph.D., University of Michigan

    Primary Reference:

    Jones JM, Huang JD, Mermall V, Hamilton BA, Mooseker MS, Escayg A, Copeland NG, Jenkins NA, Meisler MH. The mouse neurological mutant flailer expresses a novel hybrid gene derived by exon shuffling between Gnb5 and Myo5a. Hum Mol Genet. 2000 Mar 22;9(5):821-8. (Medline PMID: 10749990)

    Colony and Husbandry Information

    Careful handling during cage changing, avoid noise, mice may go into convulsions from which they will recover.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Agouti

    Other:

    MMRRC Breeding System: Sib matings

    Breeding Scheme(s):

    • Homozygous female x Homozygous male
      Heterozygous female x Heterozygous male
       

    Generation: N10+ As of: 5/2002

    Overall Breeding Performance: Slow, small litters

    Breeding Comments: Homozygous mice exhibit limb convulsions between 2-4 weeks of age, phenotype tends to lessen as mice age.

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 4 to 6

    Recommended wean age: 4 weeks - do not wean earlier due to neurological phenotype

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    000070-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.