Strain Name:
B6.Cg-Myo5aflr Gnb5flr/Mmnc
Stock Number:
000070-UNC
Citation ID:
RRID:MMRRC_000070-UNC
Other Names:
guanine nucleotide binding protein beta 5, Mysoin 5A (MyoVA)

Strain Information

Myo5aflr
Name: myosin VA; flailer
Synonyms: tb2J, flail, flr
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Gnb5flr
Name: guanine nucleotide binding protein (G protein), beta 5; flailer
Synonyms: flail, tb2J, flr
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Spontaneous Mutation
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Spontaneous Mutation
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Genetic Alterations
Due to unequal recombination within an intron of the two affected genes, the mutant chromosome contains an extra hybrid gene in addition to wildtype copies of Gnb5 and Myo5a. The flailer mice produce a hybrid protein containing the cargo-binding domain of Myosin 5a, which is expressed in neurons only. The hybrid protein acts as a dominant negative inhibitor of transport processes dependent on Myo5a.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Neurological abnormalities by 14 days postnatal include frequent falling, convulsive limb movements, opisthotonos in response to handling. Unlike the Myo5a-neurological mutants which it resembles at 2-4 weeks of age, flailer mice recover and exhibit only mild ataxia as adults. Adults do not perform well on rotorod. The histological phenotype includes delayed localization of smooth ER in dendritic spines of cerebellar Purkinje cells.
    MeSH Terms
    • Amino Acid Sequence
    • Animals
    • Base Sequence
    • Brain/cytology
    • Brain/metabolism
    • DNA, Complementary
    • Exons
    • Fungal Proteins/genetics
    • GTP-Binding Protein beta Subunits
    • Gene Dosage
    • Genes, Recessive
    • Introns
    • Mice
    • Mice, Inbred C57BL
    • Mice, Mutant Strains
    • Microscopy, Electron
    • Molecular Sequence Data
    • Monomeric GTP-Binding Proteins/genetics
    • Myosin Type I
    • Myosins/genetics
    • Purkinje Cells/metabolism
    • Purkinje Cells/ultrastructure
    • RNA, Messenger/genetics
    • RNA, Messenger/metabolism
    • Saccharomyces cerevisiae Proteins
    Strain Development
    B6CBACa-AWJ/A, tb2J/tb2J F2 mice were obtained from the frozen embryo repository at the Jackson Laboratory (Bar Harbor, ME). These mice were backcrossed once to strain C57BL/6J and heterozygous flr/+ offspring were intercrossed. flailer has subsequently been maintained by brother–sister mating of homozygous affected animals. The resulting recombinant congenic line, STOCK Myo5aflr Gnb5flr/Unc, was sib mated for at least10 generations. The composition of the background stock was ~75% C57BL/6J and 25% CBA prior to backcrossing to C57BL/6 (N>10).
    Suggested Control Mice
    • C57BL/6
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Neurologic. Can be used to study Myo5a dependent processes.
    Donor
    Miriam Meisler, Ph.D., University of Michigan
    Primary Reference
    Jones JM, Huang JD, Mermall V, Hamilton BA, Mooseker MS, Escayg A, Copeland NG, Jenkins NA, Meisler MH. The mouse neurological mutant flailer expresses a novel hybrid gene derived by exon shuffling between Gnb5 and Myo5a. Hum Mol Genet. 2000 Mar 22;9(5):821-8. (Medline PMID: 10749990)

    Colony and Husbandry Information

    Careful handling during cage changing, avoid noise, mice may go into convulsions from which they will recover.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Agouti
    MMRRC Breeding System
    Sib matings
    Breeding Scheme(s)
    • Homozygous female x Homozygous male
      Heterozygous female x Heterozygous male
       
    Generation
    N10+ As of: 5/2002
    Overall Breeding Performance
    Slow, small litters
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    4 to 6
    Recommended wean age
    4 weeks - do not wean earlier due to neurological phenotype

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000070-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.