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Strain Name:
C57BL/6J-MtgxR9756Btlr/Mmmh
Stock Number:
070001-MU
Citation ID:
RRID:MMRRC_070001-MU
Other Names:
R9756 (G1)
Major Collection:

Strain Information

Sod1
Name: superoxide dismutase 1, soluble
Synonyms: CuZnSOD, SODC, Cu/Zn-SOD, Cu(2+)-Zn2+ superoxide dismutase, Sod-1, Ipo1, Ipo-1, B430204E11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20655
VEGA: 16
Homologene: 392
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Luzp1
Name: leucine zipper protein 1
Synonyms: Luzp, 2700072H04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269593
Homologene: 11545
Zmym4
Name: zinc finger, MYM-type 4
Synonyms: 6330503C17Rik, Zfp262
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67785
Homologene: 35470
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 57,382,747 bp (GRCm38)
  • A to C, chromosome 1 at 135,386,319 bp (GRCm38)
  • G to T, chromosome 1 at 136,417,331 bp (GRCm38)
  • A to T, chromosome 1 at 190,871,824 bp (GRCm38)
  • A to G, chromosome 2 at 11,723,448 bp (GRCm38)
  • T to C, chromosome 2 at 44,997,402 bp (GRCm38)
  • C to A, chromosome 2 at 49,279,271 bp (GRCm38)
  • T to A, chromosome 2 at 85,400,338 bp (GRCm38)
  • A to T, chromosome 2 at 90,037,330 bp (GRCm38)
  • T to C, chromosome 2 at 160,960,339 bp (GRCm38)
  • G to T, chromosome 4 at 98,677,298 bp (GRCm38)
  • T to C, chromosome 4 at 107,319,050 bp (GRCm38)
  • A to G, chromosome 4 at 126,877,709 bp (GRCm38)
  • T to C, chromosome 4 at 136,542,737 bp (GRCm38)
  • A to G, chromosome 5 at 71,571,724 bp (GRCm38)
  • GC to GCGCC, chromosome 6 at 4,756,452 bp (GRCm38)
  • T to A, chromosome 6 at 87,240,954 bp (GRCm38)
  • A to G, chromosome 6 at 124,703,004 bp (GRCm38)
  • A to T, chromosome 6 at 124,728,629 bp (GRCm38)
  • A to T, chromosome 7 at 44,115,830 bp (GRCm38)
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp (GRCm38)
  • A to T, chromosome 7 at 85,619,365 bp (GRCm38)
  • A to T, chromosome 7 at 105,703,928 bp (GRCm38)
  • A to T, chromosome 7 at 106,713,795 bp (GRCm38)
  • A to G, chromosome 7 at 109,896,967 bp (GRCm38)
  • C to T, chromosome 7 at 112,303,721 bp (GRCm38)
  • T to A, chromosome 7 at 144,608,929 bp (GRCm38)
  • C to T, chromosome 8 at 21,095,927 bp (GRCm38)
  • G to A, chromosome 8 at 25,982,890 bp (GRCm38)
  • T to A, chromosome 8 at 45,043,937 bp (GRCm38)
  • T to C, chromosome 8 at 45,282,774 bp (GRCm38)
  • G to A, chromosome 8 at 99,033,344 bp (GRCm38)
  • G to A, chromosome 9 at 37,747,018 bp (GRCm38)
  • T to A, chromosome 9 at 73,932,244 bp (GRCm38)
  • T to C, chromosome 10 at 60,295,004 bp (GRCm38)
  • T to C, chromosome 10 at 62,982,740 bp (GRCm38)
  • T to C, chromosome 10 at 86,967,689 bp (GRCm38)
  • G to A, chromosome 10 at 100,516,172 bp (GRCm38)
  • T to A, chromosome 11 at 4,103,978 bp (GRCm38)
  • C to T, chromosome 11 at 61,449,841 bp (GRCm38)
  • T to A, chromosome 11 at 76,968,683 bp (GRCm38)
  • T to C, chromosome 11 at 96,303,623 bp (GRCm38)
  • T to C, chromosome 11 at 116,221,251 bp (GRCm38)
  • A to G, chromosome 12 at 76,920,164 bp (GRCm38)
  • G to A, chromosome 12 at 111,410,340 bp (GRCm38)
  • T to C, chromosome 12 at 114,253,828 bp (GRCm38)
  • A to G, chromosome 12 at 118,918,138 bp (GRCm38)
  • C to A, chromosome 13 at 65,073,600 bp (GRCm38)
  • T to C, chromosome 13 at 105,444,942 bp (GRCm38)
  • T to C, chromosome 14 at 51,162,845 bp (GRCm38)
  • C to A, chromosome 14 at 53,743,429 bp (GRCm38)
  • A to T, chromosome 14 at 55,599,125 bp (GRCm38)
  • A to G, chromosome 15 at 98,059,925 bp (GRCm38)
  • CA to C, chromosome 16 at 29,232,831 bp (GRCm38)
  • G to T, chromosome 16 at 31,944,969 bp (GRCm38)
  • T to C, chromosome 16 at 34,914,017 bp (GRCm38)
  • T to C, chromosome 16 at 38,575,764 bp (GRCm38)
  • T to G, chromosome 16 at 88,563,029 bp (GRCm38)
  • T to A, chromosome 16 at 90,220,865 bp (GRCm38)
  • A to G, chromosome 17 at 38,305,080 bp (GRCm38)
  • A to G, chromosome 17 at 43,450,246 bp (GRCm38)
  • A to G, chromosome 17 at 63,060,315 bp (GRCm38)
  • G to T, chromosome 18 at 69,657,759 bp (GRCm38)
  • G to A, chromosome 19 at 13,479,622 bp (GRCm38)
  • A to G, chromosome 19 at 42,298,341 bp (GRCm38)
  • T to C, chromosome 19 at 56,843,230 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9756 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
070001-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.