Strain Detail Sheet

Strain Name    :

C3;B6-l11Jus14/Mmucd

Stock Number :

000071-UCD

Other Names   :

Lethal 14, l11Jus14 , C3;B6-l11Jus14/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: l11Jus14
Name: lethal, Chr 11, Justice 14
Chromosome: 11; 50.5 cM
Alteration at locus: Chemically Induced
Gene Symbol: l11Jus14
Name: lethal, Chr 11, Justice 14
Chromosome: 11
Alteration at locus: Chemically Induced

Genetic Alterations:
Offspring of an ENU-induced male.

Genotype Determination:

    ES Cell Line: Not Applicable

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Hearts develop outside amnion, delayed by 8.5dpc, lethal by 10.5dpc


    Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
          assigned by genotype

    The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
    l11Jus14/l11Jus14
            Background Not Specified
    • mortality/aging
      • complete embryonic lethality during organogenesis (MGI Ref ID J:109155)
        • homozygous embryos die between 9.5 and 12.5 dpc
    • embryogenesis phenotype
      • abnormal allantois morphology (MGI Ref ID J:109155)
      • embryonic growth retardation (MGI Ref ID J:109155)
        • embryos appear as E8.5 when littermates appear as E10.5
    • growth/size phenotype
      • embryonic growth retardation (MGI Ref ID J:109155)
        • embryos appear as E8.5 when littermates appear as E10.5
    • cardiovascular system phenotype
      • abnormal heart position or orientation (MGI Ref ID J:109155)
        • hearts develop outside the amnion

    Strain of Origin: C57BL/6

    Strain genetic background: C3H

    Strain Development: For strain development, view: http://www.mouse-genome.bcm.tmc.edu/ENU/ENUexperiment.asp#Balancer

    Suggested Control Mice:

    • Wildtype littermates

    Research Applications

    • Birth Defects

    Strain Origin

    Donor: Monica J. Justice, Ph.D., Baylor College of Medicine

    Primary Reference:

    Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP, Liu B, Box N, Stockton DW, Johnson RL, Behringer RR, Bradley A, Justice MJ. Functional genetic analysis of mouse chromosome 11. Nature. 2003 Sep 4;425(6953):81-6. (Medline PMID: 12955145)

    Colony and Husbandry Information

    Special Considerations

    Genotype of cryopreserved material (sperm) is Lethal 14/Chr11-1, recommended oocyte donors are MMRRC:009973, which are Chr11-1/Re genotype. In order to determine the genotype of MMRRC:000071, a Lethal strain, it must be used in conjunction with MMRRC:009973. MMRRC:009973 is a combination of MMRRC:000055 and The Jackson Laboratory strain 000268; this combination results in a curly coated, yellow ear and yellow tail mouse which is a reporter for chromosome 11 mutations in the Lethal strains. See Mating Scheme for Chr11-1 mutants. Litters produced from resuscitation by IVF will consist of mice with the genotype Lethal 14/Chr11-1 (yellow ears and tail), L14/Re (curly hair), Chr11-1/Re (yellow ears and tail and curly hair) and Chr11-1/Chr11-1, which is embryonic lethal. You can then mate the Lethal 14/Chr11-1 to another Lethal 14/Chr11-1 and pups produced would be Lethal 14/Chr11-1, Lethal 14/Lethal 14 (embryonic lethal), and Chr11-1/Chr11-1 (also embryonic lethal.)

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Appearance

    Coat color: Agouti

    Other:

    Breeding

    MMRRC Breeding System: The MMRRC has always maintained this strain in a cryopreserved format. Information in the "Breeding" and "Reproductive statistics" sections has been provided by the Donating Investigator

    Breeding Scheme(s):

    Generation: Not applicable

    Overall Breeding Performance: Good

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: No No
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 5 - 6 pups

    Recommended wean age: 3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    000071-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    000071-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    000071-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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