Loading...

Strain Detail Sheet

Strain Name:
B6(Cg)-Tmem106btm2d(KOMP)Wtsi/JjanMmjax
Stock Number:
071637-JAX
Citation ID:
RRID:MMRRC_071637-JAX
Other Names:
TMEM106B tm2d

Strain Information

Gene Details

Tmem106btm2d(KOMP)Wtsi
Name: transmembrane protein 106B; targeted mutation 2d, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Tmem106b
Name: transmembrane protein 106B
Synonyms: 6430519M21Rik, 5830455K21Rik, 2310036D22Rik
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 71900
Homologene: 56806

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Deletion of Tmem106b via flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s).
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
Deletion of TMEM106B has limited phenotype on its own, but exacerbates accumulation of phosphorylated tau, accelerates neurological phenotypes, and worsens transcriptional signatures of disease in a mouse model of Alzheimer's/frontotemporal dementia.
MeSH Terms
  • Animals
  • Humans
  • Mice
  • Disease Models, Animal
  • Membrane Proteins/genetics
  • Mice, Knockout
  • Mice, Transgenic
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Paralysis/genetics
  • Polymorphism, Single Nucleotide
  • tau Proteins/genetics
  • tau Proteins/metabolism
  • Tauopathies/pathology
Strain Development
The strain was derived from the germplasm containing the allele C57BL/6N-Tmem106btm2a(KOMP)Wtsi, which we used for IVF of C57BL/6J oocytes. Founder offspring were successively mated with beta-actin-Cre and beta-actin-Flp transgenic lines to delete the loxP-flanked exon 4 and the frt-flanked neo-lacZ cassette. We have bred out the Flp and Cre alleles and maintained the line on a C57BL/6J background by successive generations of heterozygous backcross to C57BL/6J wild-type mates. At least one generation included breeding Ht female with WT male to render the Y chromosome fully C57BL/6J.


Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Neurobiology

Strain Origin

Donor
Joanna Jankowsky, Ph.D., Baylor College of Medicine.
Primary Reference

Edwards GA 3rd, Wood CA, He Y, Nguyen Q, Kim PJ, Gomez-Gutierrez R, Park KW, Xu Y, Zurhellen C, Al-Ramahi I, Jankowsky JL. TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy. Acta Neuropathol. 2024 Mar 25;147(1):61. doi: 10.1007/s00401-024-02701-5. (Medline PMID: 38526616)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N9
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.