Strain Name:
Nos1ap tm1c
Stock Number:
071662-MU
Citation ID:
RRID:MMRRC_071662-MU
Other Names:
Nos1ap tm1c

Strain Information

Nos1ap
Name: nitric oxide synthase 1 (neuronal) adaptor protein
Synonyms: 6330408P19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70729
Homologene: 135990
Genetic Alterations
Cre-recombinase conditional knockout (exon 4 floxed). The Cre-recombinase conditional knockout allele (tm1c) is derived from the reporter-tagged knockout allele with conditional potential (tm1a), generated by KOMP.
ES Cell Line
JM8A3.N1
Phenotype
Heart-restricted, partial loss of expression of Nos1ap led to changes in cardiac electrophysiology.
MeSH Terms
  • Animals
  • Mice
  • Adaptor Proteins, Signal Transducing/genetics
  • Electrocardiography
  • Genome-Wide Association Study
  • Genotype
  • Myocardium
  • Polymorphism, Single Nucleotide
  • Zebrafish/genetics
Strain Development
Targeted mouse embryonic (ES) cells (EPD0804_4_E03 and EPD0804_4_G02) with Nos1ap ‘knockout-first allele’ (Nos1aptm1a; reporter-tagged insertion with conditional potential) (Skarnes et al. 2011) derived from the parental ES cell line JM8A3.N1 (A/a; Tyr+/+) of the C57BL/6N strain (Pettitt et al. 2009) were purchased from the Knockout Mouse Project (KOMP) repository. Injections into albino blastocysts (C57BL/6 a/a; Tyrc/c) and generation of G0 chimeras with agouti coat color was performed at Texas A&M Institute of Genomic Medicine. Chimeric mice were crossed to C57BL/6J (a/a; Tyr+/+; Jax stock #000664) and G1 mice were genotyped by PCR to assess germline transmission. Nos1ap+/tm1a mice were crossed with ACTB-Flpe (Jax stock #005703) (Rodríguez et al. 2000) to generate Cre-recombinase conditional knockout mice (lacZ-deleted neoR-deleted, Nos1ap-exon4 floxed; Nos1ap+/tm1c or Nos1ap+/fl). Nos1ap tm1c allele was maintained by backcrossing to C57BL/6J.
Suggested Control Mice
Wildtype C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Models for Human Disease
  • Research Tools
Donor
Ashish Kapoor, Ph.D., The University of Texas Health Science Center at Houston (UTHealth).
Primary Reference

Smith A, Auer D, Johnson M, Sanchez E, Ross H, Ward C, Chakravarti A, Kapoor A. Cardiac muscle-restricted partial loss of Nos1ap expression has limited but significant impact on electrocardiographic features. G3 (Bethesda). 2023 Nov 1;13(11):jkad208. doi: 10.1093/g3journal/jkad208. (Medline PMID: 37708408)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
30
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071662-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
071662-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.