Loading...

Strain Detail Sheet

Strain Name:
STOCK Grk1tm1.1Elrw/Mmnc
Stock Number:
071686-UNC
Citation ID:
RRID:MMRRC_071686-UNC
Other Names:
GRK1-S21A knock-in

Strain Information

Gene Details

Grk1tm1.1Elrw
Name: G protein-coupled receptor kinase 1; targeted mutation 1.1, Ellen R Weiss
Synonyms: GRK1-Ser21A
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knock-In
Grk1
Name: G protein-coupled receptor kinase 1
Synonyms: RK, Rhok
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knock-In
NCBI: 24013
Homologene: 2197

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Serine, codon 21 (AGT) in exon 1, was changed to alanine (p.S21A) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation prevents phosphorylation of the affected residue in the encoded peptide.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    C57BL/6N-PRX-B6N #1 derived from C57BL/6N

    Strain Description

    Phenotype
    Rod dark adaptation following exposure to bright light was significantly delayed. This suggests that the increased activity of the kinase results in enhanced rhodopsin phosphorylation and thus delays its regeneration.
    MeSH Terms
    • Animals
    • Dark Adaptation/physiology
    • G-Protein-Coupled Receptor Kinase 1/genetics
    • G-Protein-Coupled Receptor Kinase 1/metabolism
    • Kinetics
    • Mice, Transgenic
    • Phosphorylation
    • Retina/metabolism
    • Retinal Cone Photoreceptor Cells/metabolism
    • Retinal Rod Photoreceptor Cells/metabolism
    • Rhodopsin/metabolism
    • Serine/metabolism
    Strain Development
    GRK1-S21A knock-in mice were generated by homologous recombination in C57BL/6N ES cells. A gene targeting vector including a mutation of serine 21 to alanine in exon 1 and a loxP-flanked PGK-Neomycin resistance cassette in intron 1 was constructed and electroporated into C57BL/6N-PRX-B6N ES cells. Correctly targeted cells were injected into C57BL/6-albino blastocysts for chimera formation. Chimeras were mated to C57BL/6-albino animals for germline transmission of the targeted allele. Positive mice were mated to B6.C-Tg(CMV-cre)1Cgn/J, to remove the floxed neo cassette. The resulting line containing the S21A mutation was backcrossed to homozygosity and crossed into C57BL/6J mice to ensure the absence of the rd8 mutation.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cell Biology
    • Internal/Organ
    • Neurobiology

    Strain Origin

    Donor
    Ellen Weiss, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Kolesnikov AV, Chrispell JD, Osawa S, Kefalov VJ, Weiss ER. Phosphorylation at Serine 21 in G protein-coupled receptor kinase 1 (GRK1) is required for normal kinetics of dark adaption in rod but not cone photoreceptors. FASEB J. 2020 Feb;34(2):2677-2690. doi: 10.1096/fj.201902535R. Epub 2019 Dec 30. (Medline PMID: 31908030)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    N/A
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Undetermined
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Undetermined

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071686-UNC-HOM-F
    071686-UNC-HOM-M
    		Homozygous Female
    Homozygous Male
    		 $311.00 / $516.00
    Non-Profit / For-Profit    		 Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.