Loading...

Strain Detail Sheet

Strain Name:
STOCK Grk1tm2Elrw/Mmnc
Stock Number:
071688-UNC
Citation ID:
RRID:MMRRC_071688-UNC
Other Names:
GRK1-S21E knock in

Strain Information

Gene Details

Grk1
Name: G protein-coupled receptor kinase 1
Synonyms: RK, Rhok
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 24013
Homologene: 2197

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Serine codon 21 (AGT) in exon 1 was changed to glutamic acid (p.S21E) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent Cre-mediated recombination.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    PRX-B6N #1

    Strain Description

    Phenotype
    The ability to phosphorylate rhodopsin is reduced in S21E. WT GRK1 treated with forskolin behaves similarly to the S21E mutant. The mutation prevents phosphorylation of the affected residue in the encoded peptide. To be clear, this strain does not form a functional Grk1 protein and does not have Rd8 retinal degeneration present in C57BL/6N mice.
    Strain Development
    GRK1-S21E knock-in mice were generated by homologous recombination in C57BL/6N ES cells. A gene targeting vector was constructed by recombineering using a BAC clone containing the full-length mouse gene for Grk1. The targeting vector included a mutation of serine 21 to glutamic acid in exon 1 and a loxP-flanked PGK-Neomycin resistance cassette in intron 1. The targeting vector was electroporated into C57BL/6N-PRX-B6N ES cells and clones were screened by PCR and Southern blot analysis. The presence of the S21E mutation was confirmed in clones by PCR amplification and digestion with SfoI, a novel restriction enzyme site created by the mutation. Correctly targeted cells were injected into C57BL/6-albino blastocysts for chimera formation. Chimeras were mated to C57BL/6-albino animals for germline transmission of the targeted allele. Insertion of the mutant DNA into the germline was further validated by PCR screening. Positive mice were mated to a Cre deleter line, B6.C-Tg(CMV-cre)1Cgn/J, to remove the floxed Neo-cassette. Finally, the resulting line containing the S21E mutation was backcrossed to homozygosity and crossed into C57Bl/6J mice to ensure the absence of the rd8 mutation.
    Suggested Control Mice
    WT C57BL/6J and GRK1-/-

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cell Biology
    • Internal/Organ
    • Neurobiology

    Strain Origin

    Donor
    Ellen Weiss, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference
    Not ready to publish

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    N/A
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Undetermined
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Undetermined

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071688-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    071688-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.