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Strain Detail Sheet

Strain Name:
B6J.B6N-Myo10tm1c(KOMP)Wtsi/Mmnc
Stock Number:
071697-UNC
Citation ID:
RRID:MMRRC_071697-UNC
Other Names:
Myo10tm1c

Strain Information

Gene Details

Myo10tm1c(KOMP)Wtsi
Name: myosin X; targeted mutation 1c, Wellcome Trust Sanger Institute
Synonyms: Myo10tm1c
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Targeted Mutation
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The L1L2_Bact_P cassette was inserted at position 25785401 of Chromosome 15 upstream of the critical exon (exon 27) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 27 at position 25786400. Exon 27 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 27.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8A1.N3 derived from C57BL/6N-Atm1Brd

    Strain Description

    Phenotype

    Conditional phenotype: The Myo10tm1c allele has loxP sites flanking exon 27 to allow cre-mediated deletion of exon 27. Deleting exon 27 results in truncation and loss of full-length Myo10 as well as headless Myo10. Headless Myo10 is generated by use of alternative transcription start sites in intron 19 and typically includes exons 20-41 of full length Myo10.

    Approximately half of homozygous Myo10 KO mice exhibit the lethal embryonic defect of exencephaly, but KO mice that survive birth are able to grow and breed relatively normally. Heterozygous KO mice appear similar to WT mice.


    MeSH Terms
    • Animals
    • Female
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Myosins/physiology
    • Neovascularization, Pathologic
    • Neural Tube/physiopathology
    • Ophthalmic Artery/metabolism
    • Ophthalmic Artery/physiopathology
    • Pigmentation
    • Pseudopodia/metabolism
    • Pseudopodia/pathology
    • Vitreous Body/blood supply
    • Vitreous Body/metabolism
    • Vitreous Body/pathology
    Strain Development
    KOMP Myo10tm1a "KO-first" ES cells (EPD0332_3-B02 cells derived from JMA3.N1 ES cells, a cell line derived from a male C57BL/6N-Atm1Brd mouse; see KOMP project 50217) were injected into blastocysts and the resulting chimeras were bred to albino C57BL/6 mice to get germline transmission on a C57BL/6 background. The resulting germline were crossed with mice expressing FLPo recombinase to remove the "KO-first" cassette while retaining the floxed exon 27 to produce the Myo10tm1c allele. These mice were crossed with one another and with C57BL/6J mice. A colony was established by breeding them to one another prior to backcrossing to make the strain congenic to C57BL/6J (Jackson Strain #000664).

    MiniMUGA testing in 2022 showed 99.9% C57BL/6J and the colony was then maintained by intra-strain mating of homozygotes till deposit.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Cardiovascular
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Immunology and Inflammation
    • Internal/Organ
    • Models for Human Disease
    • Neurobiology
    • Reproduction
    • Sensorineural

    Strain Origin

    Donor
    Richard Cheney, Ph.D., UNC Chapel Hill School of Medicine.
    Primary Reference

    Heimsath EG Jr, Yim YI, Mustapha M, Hammer JA, Cheney RE. Myosin-X knockout is semi-lethal and demonstrates that myosin-X functions in neural tube closure, pigmentation, hyaloid vasculature regression, and filopodia formation. Sci Rep. 2017 Dec 11;7(1):17354. doi: 10.1038/s41598-017-17638-x. (Medline PMID: 29229982)

    Colony and Husbandry Information

    Special Considerations

    none

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    not certain; minimuga testing in 2022 showed 99.9% C57BL/6J.
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071697-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.