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Strain Detail Sheet

Strain Name:
B6J.129S1-Portm1Ding/Mmucd
Stock Number:
071733-UCD
Citation ID:
RRID:MMRRC_071733-UCD
Other Names:
CPR2lox B6N10

Strain Information

Gene Details

Portm1Ding
Name: cytochrome p450 oxidoreductase; targeted mutation 1, Xinxin Ding
Synonyms: Cprlox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exons 3 through 15 were flanked by single loxP sites located in introns 2 and 15.
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

Strain Description

Phenotype

Homozygous Cprlox mice are fertile and without any histological abnormality or any change in CPR expression.
Conditional phenotype: yes, floxed

MeSH Terms
  • Alleles
  • Animals
  • Blotting, Southern
  • Disease Models, Animal
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Enzymologic
  • Homozygote
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mice, Knockout
  • Mice, Transgenic
  • Microsomes, Liver/enzymology
  • Microsomes, Liver/metabolism
  • NADPH-Ferrihemoprotein Reductase/genetics
  • NADPH-Ferrihemoprotein Reductase/metabolism
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Tissue Distribution
  • Basic Helix-Loop-Helix Transcription Factors/agonists
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cytochrome P-450 Enzyme System/genetics
  • Cytochrome P-450 Enzyme System/metabolism
  • Growth Hormone/metabolism
  • Inflammation Mediators/metabolism
  • Isoenzymes
  • Janus Kinase 2/drug effects
  • Janus Kinase 2/metabolism
  • Liver/drug effects
  • Liver/enzymology
  • Methylcholanthrene/pharmacology
  • Mice, Inbred C57BL
  • Microsomes, Liver/drug effects
  • NADPH-Ferrihemoprotein Reductase/deficiency
  • RNA, Messenger/metabolism
  • Receptors, Aryl Hydrocarbon/agonists
  • Receptors, Aryl Hydrocarbon/metabolism
  • Receptors, Somatotropin/drug effects
  • Receptors, Somatotropin/genetics
  • Receptors, Somatotropin/metabolism
  • STAT5 Transcription Factor/genetics
  • STAT5 Transcription Factor/metabolism
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Time Factors
  • Cytochromes b5/metabolism
  • Pharmaceutical Preparations/metabolism
  • Tumor Cells, Cultured
  • Base Sequence
  • DNA Primers
  • DNA Probes
  • Intestines/enzymology
  • RNA, Messenger/genetics
Strain Development
A Cpr genomic clone was isolated from a 129/SvJ mouse BAC genomic library. CJ7 ES cells originating from 129S1/Sv-Oca2+ Tyr+ Kitl+ were used for electroporation. ES cell clones with confirmed homologous recombination were electroporated and analyzed. Homologous recombinant ES cells were injected into C57BL/6J blastocysts. Bastocysts were implanted into pseudopregnant B6/CBA F1 mice to generate offspring. Male chimeras were bred with wild-type C57BL/6J mice to obtain germline transmission F1 mice that are heterozygous for the mutant allele. The F2 homozygotes were obtained by intercrossing the F1 heterozygotes. Mice were backcrossed to C57BL/6 for atleast 10 generations.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Metabolism
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor
Xinxin Ding, Ph.D., University of Arizona.
Primary Reference

Wu L, Gu J, Weng Y, Kluetzman K, Swiatek P, Behr M, Zhang QY, Zhuo X, Xie Q, Ding X. Conditional knockout of the mouse NADPH-cytochrome p450 reductase gene. Genesis. 2003 Aug;36(4):177-81. doi: 10.1002/gene.10214. (Medline PMID: 12929087)

Lee C, Ding X, Riddick DS. The role of cytochrome P450-dependent metabolism in the regulation of mouse hepatic growth hormone signaling components and target genes by 3-methylcholanthrene. Drug Metab Dispos. 2013 Feb;41(2):457-65. doi: 10.1124/dmd.112.048835. Epub 2012 Nov 20. (Medline PMID: 23169610)

Riddick DS, Ding X, Wolf CR, Porter TD, Pandey AV, Zhang QY, Gu J, Finn RD, Ronseaux S, McLaughlin LA, Henderson CJ, Zou L, Flück CE. NADPH-cytochrome P450 oxidoreductase: roles in physiology, pharmacology, and toxicology. Drug Metab Dispos. 2013 Jan;41(1):12-23. doi: 10.1124/dmd.112.048991. Epub 2012 Oct 19. (Medline PMID: 23086197)

Cheng X, Gu J, Klaassen CD. Adaptive hepatic and intestinal alterations in mice after deletion of NADPH-cytochrome P450 Oxidoreductase (Cpr) in hepatocytes. Drug Metab Dispos. 2014 Nov;42(11):1826-33. doi: 10.1124/dmd.114.060053. Epub 2014 Aug 21. (Medline PMID: 25147274)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N=10
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071733-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.