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Strain Detail Sheet

Strain Name:
C57BL/6-Cyp2s1tm1Ding/Mmucd
Stock Number:
071818-UCD
Citation ID:
RRID:MMRRC_071818-UCD
Other Names:
Cyp2s1-Null

Strain Information

Gene Details

Cyp2s1tm1Ding
Name: cytochrome P450, family 2, subfamily s, polypeptide 1; targeted mutation 1, Xinxin Ding
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Cyp2s1
Name: cytochrome P450, family 2, subfamily s, polypeptide 1
Synonyms: 1200011C15Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 74134
Homologene: 75274

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exon 3 was replaced with a hygromycin resistance gene cassette and loxP site via homologous recombination.
ES Cell Line
Bruce 4 derived from B6.Cg-Thy1a

Strain Description

Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Base Sequence
  • Cytochrome P-450 Enzyme System/genetics
  • Cytochrome P-450 Enzyme System/metabolism
  • DNA Primers
  • Mice
  • Mice, Knockout
  • Models, Animal
  • Multigene Family
  • Polymerase Chain Reaction
Strain Development
Exon 3 of Cyp2s1 was replaced with a hygromycin-resistance gene, together with a loxp site via homologous recombination. The targeting vector containing the 1.4-kb 5′ homology region, Cyp2s1 exon 1~2, the hygromycin B resistance gene (hygro), a loxP site, Cyp2s1 exon 4~9 and the 7.0-kb 3′ homology region was electroporated into Bruce 4 (B6.Cg-Thy1a) ES cells. Positive ES cell clones were then injected into the blastocysts from albino B6(Cg)-Tyrc-2J/J (stock 000058; The Jackson Laboratory, Bar Harbor, ME) female mice. The blastocysts were transferred into the uterus of a pseudopregnant B6CBAF1/J mouse to generate offspring. Adult chimeras were bred with WT C57BL/6 female mice to obtain germline transmission F1 mice that were heterozygous for the mutant allele. Homozygous Cyp2s1-null mice were generated by intercrossing the Cyp2s1+/− heterozygotes.
Suggested Control Mice
B6

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Metabolism
  • Research Tools

Strain Origin

Donor
Xinxin Ding, Ph.D., University of Arizona.
Primary Reference

Wei Y, Li L, Zhou X, Zhang QY, Dunbar A, Liu F, Kluetzman K, Yang W, Ding X. Generation and characterization of a novel Cyp2a(4/5)bgs-null mouse model. Drug Metab Dispos. 2013 Jan;41(1):132-40. doi: 10.1124/dmd.112.048736. Epub 2012 Oct 16. (Medline PMID: 23073733)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071818-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit		 Aliquot Approximate quantity3
071818-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.