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Strain Detail Sheet

Strain Name:
C57BL/6J-Armcx1tm1Sgos/Mmnc
Stock Number:
071840-UNC
Citation ID:
RRID:MMRRC_071840-UNC
Other Names:
Armcx1 loxP/loxP

Strain Information

Gene Details

Armcx1
Name: armadillo repeat containing, X-linked 1
Synonyms: 3010033I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 78248
Homologene: 9589

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exon 6 (containing the entire coding region) of Armcx1 is flanked by loxP sites.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8A3.N1 derived from C57BL/6N

    Strain Description

    Phenotype

    Homozygous and heterozygous mice have no ocular phenotype detected and no comprehensive morphological analysis.

    Conditional Phenotype: Crossing this floxed model with a global cre driver (bActin-Cre) to create a germline knockout of Armcx1 resulted in no ocular phenotype.

    Strain Development
    JM8A3.N1 mouse ES cells were electroporated using linearized Armcx1 targeting vector DNA. 336 neomycin (G418) resistant ES clones were picked and analyzed by PCR. ES clones #15, #30 and #212 were identified as correctly targeted at the Armcx1 locus by PCR and confirmed by Southern blot analysis. ES Clones #15 and #212 were selected for in vitro deletion of FRT floxed PGK-Neo cassette by electroporating pCAGGS-Flpe-Puromycin plasmid and selected for puromycin (1.5ug/ml) resistance. For ES clones #15 and #212, 44 and 37 clones respectively, were isolated and subjected to PCR analysis for absence of PGK-Neo cassette and tested for neomycin sensitivity. For ES clone #15 and #212, 13/44 clones and 11/37 clones respectively, were both negative for PGK-Neo cassette and neomycin sensitive. Two clones from each #15 and #212 PGK-Neo deleted were selected for karyotyping. ES cell lines were injected into blastocysts harvested after mating C57BL/6Hsd (Envigo) females to generate chimeric lines. Chimeras were backcrossed to test germline transmission.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Metabolism
    • Neurobiology

    Strain Origin

    Donor
    Sidney Gospe, M.D., Duke University Medical Center.
    Primary Reference

    Submitted to Genesis (minor revisions in process)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N=1
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071840-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.