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Strain Detail Sheet

Strain Name:
C57BL/6J-Cd320tm1Lcb/Mmnc
Stock Number:
071880-UNC
Citation ID:
RRID:MMRRC_071880-UNC
Other Names:
C57BL/6J Cd320tm1Lcb

Strain Information

Gene Details

Cd320
Name: CD320 antigen
Synonyms: NG29, D17Ertd716e, 8D6, VLDL, 425O18-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 54219
Homologene: 9573

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This allele harbors the entire Cd320 gene with exons 1 and 2 flanked with loxp sites (floxed) and a third loxp site flanking the PGK-NEO gene inserted in intron 2.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    HGTC-8 derived from C57BL/6

    Strain Description

    Phenotype
    None/Normal/Wild-type

    Conditional phenotype: no

    MeSH Terms
    • Anemia/etiology
    • Animals
    • Diet
    • Disease Models, Animal
    • Female
    • Male
    • Mice
    • Mice, Knockout
    • Receptors, Cell Surface/genetics
    • Reproduction
    • Vitamin B 12 Deficiency/complications
    • Vitamin B 12 Deficiency/genetics
    • Vitamin B 12 Deficiency/metabolism
    • Vitamin B 12 Deficiency/physiopathology
    Strain Development
    The Cd320 gene was targeted in HGTC-8 mouse embryonic stem cells using the Cre-loxP system. The Cd320tm1Lcb targeted mice were engineered to carry a conditional deletion of the first two of the five exons in the Cd320 gene by flanking exons 1 and 2 with loxP sites. This region targeted for deletion contains the transcription start codon in exon 1 and is predicted to delete the first 83 amino acids of the protein including the sequences for the transmembrane targeting signal and the first LDL-A repeat domain. The Neomycin selection construct is inserted into the intron 2 of the Cd320 gene and is flanked by a third loxp site. The targeting vector was electroporated into the HGTC-8 ES cells derived from C57BL/6J. 240 ES cell colonies were cloned, and 11 homologously targeted clones were identified by Southern blotting. Two clones were expanded and injected into Balb/C blastocysts. These C57BL/6J Cd320tm1Lcb mice on a pure C57BL/6J background were bred to homozygosity, having both alleles flanked by loxP sites (floxed). These mice retain the Neo selection cassette and the three loxp sites.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Developmental Biology
    • Hematology
    • Metabolism
    • Models for Human Disease
    • Reproduction
    • Sensorineural

    Strain Origin

    Donor
    Lawrence Brody, Ph.D., National Human Genome Research Institute.
    Primary Reference

    Bernard DJ, Pangilinan FJ, Cheng J, Molloy AM, Brody LC. Mice lacking the transcobalamin-vitamin B12 receptor, CD320, suffer from anemia and reproductive deficits when fed vitamin B12-deficient diet. Hum Mol Genet. 2018 Oct 15;27(20):3627-3640. doi: 10.1093/hmg/ddy267. (Medline PMID: 30124850)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    2
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Anna Solowiej.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Anna Solowiej

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071880-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / $5,382.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.