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Strain Detail Sheet

Strain Name:
B6;129S5-S100a8tm1Lex/Mmnc
Stock Number:
071886-UNC
Citation ID:
RRID:MMRRC_071886-UNC
Other Names:
S100A8-/-

Strain Information

Gene Details

S100a8tm1Lex
Name: S100 calcium binding protein A8 (calgranulin A); targeted mutation 1, Lexicon Pharmaceuticals
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
S100a8
Name: S100 calcium binding protein A8 (calgranulin A)
Synonyms: MRP8, Caga, 60B8Ag, CFAg, CP-10, B8Ag, p8
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 20201
Homologene: 2225

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A 529 bp region containing exons 2 and 3 was replaced by a LacZ/neomycin selection cassette.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Lex-1

    Strain Description

    Phenotype
    S100a8-/- mice have increased numbers of neutrophils, monocytes and dendritic cells in the blood and bone marrow, and these all expressed myeloid markers such as CD11b, Ly6G and CD86 more strongly. Granulocyte-macrophage common precursors were increased in S100a8-/- bone marrow and yielded greater numbers of macrophages and dendritic cells in culture. The animals also developed more severe arthritic disease leading to aggravated osteoclast activity and bone destruction.
    MeSH Terms
    • Animals
    • Arthritis, Experimental/diagnostic imaging
    • Arthritis, Experimental/pathology
    • Bone Marrow/pathology
    • Bone and Bones/diagnostic imaging
    • Bone and Bones/pathology
    • Calgranulin A/deficiency
    • Calgranulin A/metabolism
    • Cartilage/pathology
    • Cell Differentiation
    • Dendritic Cells/metabolism
    • Female
    • Gene Deletion
    • Mice
    • Myeloid Cells/pathology
    • Myelopoiesis
    Strain Development
    The S100a8 targeting vector was derived using the Lambda KOS system. The Lambda KOS phage library was screened by PCR using primers specific for exons 2 and 3. pKOS genomic clones (pKOS-18, pKOS-23 and pKOS-64) were isolated from the library and confirmed by sequence and restriction analysis. Gene-specific arms were appended by PCR to a yeast selection cassette containing the URA3 marker. The yeast selection cassette and pKOS-18 were co-transformed into yeast. Clones that had undergone homologous recombination to replace a 529 bp region containing exons 2 and 3 with the yeast selection cassette were isolated. The yeast cassette was subsequently replaced with a LacZ/Neo selection cassette to complete the S100a8 targeting vector. The linearized targeting vector was electroporated into 129/SvEvBrd (Lex-1) ES cells. Correctly targeted clones were identified and confirmed by Southern blot analysis. Three targeted ES cell clones were identified and microinjected into C57BL/6 (albino) blastocysts to generate chimeric animals that were bred to C57BL/6 (albino) females, and the resulting heterozygous offspring were interbred to produce homozygous S100a8-deficient mice. The genotype at the S100a8 locus was determined by screening for the Neo cassette via PCR.
    Suggested Control Mice
    C56BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Philippe Tessier, Ph.D., CHU de Quebec Universite Laval.
    Primary Reference

    Cesaro A, Defrêne J, Lachhab A, Pagé N, Tardif MR, Al-Shami A, Oravecz T, Fortin PR, Daudelin JF, Labrecque N, Aoudjit F, Pelletier M, Tessier PA. Enhanced myelopoiesis and aggravated arthritis in S100a8-deficient mice. PLoS One. 2019 Aug 22;14(8):e0221528. doi: 10.1371/journal.pone.0221528. (Medline PMID: 31437241)

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    Heterozygous offspring were interbred to produce homozygous mice.
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Universite Laval - BLUM.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Universite Laval - BLUM

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071886-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.