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Strain Detail Sheet

Strain Name:
C57BL/6N-Tg(CD2-Tcra,-Tcrb)1Ybe/Mmjax
Stock Number:
072057-JAX
Citation ID:
RRID:MMRRC_072057-JAX
Other Names:
SA1 Tg

Strain Information

Gene Details

Tcrb
Name: T cell receptor beta chain
Synonyms: Tib, TCRbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Tcra
Name: T cell receptor alpha chain
Synonyms: Tcr alpha, Tcralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
CD2
Name: CD2 molecule
Type: Gene
Species: Homo sapiens (human)
Chromosome: 1
Tg(CD2-Tcra,-Tcrb)1Ybe
Name: transgene insertion 1, Yasmine Belkaid
Synonyms: SA1-Tg, Tg(CD2-Tcr*)1Ybe
Type: Transgene
Species: Multi-species
Chromosome: Unknown
Alteration at locus: Transgenic
Genetic Alterations
SA1-Tg mice express T cell receptor α and β chains, driven by a human CD2 promoter, that recognize components of the common human skin commensal Staphylococcus aureus.

Note that the donating investigator has also made an SA2-Tg line available as MMRRC RRID:072060.
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
None/Normal/Wild-type. SA1-transgenic (Tg) mice express T cell receptor α and β chains, driven by a human CD2 promoter, that recognize components of the common human skin commensal Staphylococcus aureus. Hemizygous mice are viable and fertile with no overt phenotype. The donating laboratory has not attempted to generate homozygous mice. The SA1-Tg and SA2-Tg mouse lines (MMRRC RRID:072060) are S. aureus-specific transgenic mice where all CD4+ T cells are specific for components of S. aureus 42F02. However, each strain has a unique TCR construct and likely will not recognize the same S. aureus antigen. These mice may be useful for tracking S. aureus-specific CD4+ T cells in vivo.
MeSH Terms
  • Axons
  • Interleukin-17
  • Microbiota
  • Nerve Regeneration/physiology
  • Sensory Receptor Cells
  • Animals
  • Mice
  • Th17 Cells/cytology
Strain Development
To generate the SA1-transgene (Tg), Foxp3-GFP reporter mice on a C57BL/6 background, were topically associated with S. aureus (42F02), and 4 weeks after the first association, mice were infected with S. aureus (42F02) by an intradermal injection in the ear to recall the S. aureus-specific T cells. An s. aureus-specific T cell suspension was obtained from the ear skin-draining lymph nodes and co-cultured with S. aureus loaded dendritic cells for several weeks. FoxP3-D4+CCR6+ T cells were FACSorted from the in vitro culture and subjected to single-cell sequencing of TCRα and TCRβ chains. Clonal TCR pairs (differing from the TCR pair used for creating SA2-Tg mice, MMRRC RRID:072060) were identified and used in a hybridoma reconstitution screening assay to identify S. aureus-reactive TCR heterodimers. A single S. aureus-specific TCR pair was cloned into a human CD2-expression vector. The resulting SA1-Tg construct was injected into the pronuclei of fertilized C57BL/6NCr oocytes. Founder line 1 was established and backcrossed at least 6 generations to C57BL/6NTac by the donating laboratory. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes.

Of note, the transgene insertion site and/or copy number are not known.

Suggested Control Mice
C57BL/6

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Yasmine Belkaid, Ph.D., National Institutes of Health, National Institute of Allergy and Infectious Diseases.
Primary Reference

Enamorado M, Kulalert W, Han SJ, Rao I, Delaleu J, Link VM, Yong D, Smelkinson M, Gil L, Nakajima S, Linehan JL, Bouladoux N, Wlaschin J, Kabat J, Kamenyeva O, Deng L, Gribonika I, Chesler AT, Chiu IM, Le Pichon CE, Belkaid Y. Immunity to the microbiota promotes sensory neuron regeneration. Cell. 2023 Feb 2;186(3):607-620.e17. doi: 10.1016/j.cell.2022.12.037. Epub 2023 Jan 13. (Medline PMID: 36640762)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Other or uncertain
Generation
N/A
Overall Breeding Performance
Poor

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: No Yes
Age Reproductive Decline: Less than 4 months Less than 4 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
072057-JAX-HET-F
072057-JAX-HET-M
072057-JAX-WT-F
072057-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
 $229.00 / $229.00
Non-Profit / For-Profit		 Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.