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Acta2 codes for the structural protein, smooth muscle alpha (α)-2 actin, which is essential for smooth muscle structure and contractility. Acta2-R179Hfl mice harbor a Cre-inducible R179H mutation in exon 6 of the Acta2 gene. Homozygous mice are viable and fertile with no overt phenotype. After Cre-mediated recombination, ACTA2 with the R179H missense mutation is expressed in cre-expressing tissues.After crossing these Acta2-R179Hfl mice to B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J (JAX Stock No. 007742) mouse line, the resulting progeny expressing the R179H mutation, demonstrated bladder, bowel, and vascular dysfunction by 6-12 weeks of age. These Cre-inducible Acta2-R179Hfl mice may be useful for studying vascular diseases as well as multisystem smooth muscle dysfunction syndrome (MSDS).
Conditional phenotype: Yes, the model is cre inducible.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Zimmerman, R A et al. “Decreased expression of smooth muscle alpha-actin results in decreased contractile function of the mouse bladder.” The Journal of urology vol. 172,4 Pt 2 (2004): 1667-72. doi:10.1097/01.ju.0000139874.48574.1b
The Acta2-R179Hfl knock-in allele was designed to incorporate a conditional R179H (CGT to CAT) mutation into exon 6 of the actin alpha 2, smooth muscle, aorta (Acta2) gene. The targeting vector consists of an initial loxP site immediately upstream of the endogenous splice acceptor site of intron 4, coding sequence of exon5-6, an SV40 polyA sequence, a neomycin (neo) cassette flanked by self-deletion anchor sites (SDA) and diphtheria toxin A (DTA), used for negative selection, and a second loxP site. The R179H (CGT to CAT) mutation in exon 6 is located downstream of the second loxP site. The target construct was electroporated into C57BL/6N-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 albino blastocysts, which were then re-implanted into CD1 recipient females. The resulting chimeric mice were bred to C57BL/6NCrl mice to confirm germline transmission. The donating laboratory maintained this mouse line on a C57BL/6NCrl background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes.
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Musolino Lab.
A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Musolino Lab
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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