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Strain Detail Sheet

Published: 09/19/2025
Revised: 09/25/2025

Strain Name:
B6J.129S1(Cg)-Dp(19Rln1-Gldc)3Uru/Mmmh
Stock Number:
072077-MU
Citation ID:
RRID:MMRRC_072077-MU
Other Names:
B6J.Cg(129S1)-Gldc3Uru

Strain Information

Gene Details

Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141

Additional Resources
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Genetic Alterations
This strain harbors a duplication of the Gldc gene. Two loxP sites have been inserted flanking the Gldc gene, one using gene targeting in ES cells, the other with CRISPR/Cas-9 in zygotes. Mice carrying the two loxP sites in trans and a Hprt-Cre transgene were bred with wild type mice to identify mice that have undergone trans-allelic recombination, effectively duplicating the Gldc gene.
ES Cell Line
V6.5

Strain Description

Phenotype
The Rln1-Gldc chromosomal segment is duplicated/triplicated in patients with schizoaffective disorder and bipolar disorder with psychotic features. This strain contains a duplication of the Gldc gene encoding the glycine decarboxylase gene, a glycine-degrading enzyme.
  • Homozygous mice: contain 4 copies of Gldc instead of the normal 2 copies
    • Glycine decarboxylase expression and activity in the brain are increased
    • miR-137 expression are increased (strongly associated with schizophrenia in GWAS) and Pyroxd2 expression
    • BDNF expression and Akt/mTOR signaling is decreased
    • Total glycine concentrations from hippocampal or prefrontal cortex is unaltered, the extracellular glycine concentration is reduced in dentate gyrus (DG) but not in CA1
    • LTP deficit at mPP-DG synapses, but not at CA3-CA1 synapses
    • Baseline and FCCP-stimulated mitochondrial respiration are reduced in DG but not in CA1
    • Behaviorally, mice display a startle habituation deficit, a latent inhibition deficit, working memory deficits in the Y maze and the water T maze, and both sociability and social preference deficits in the three-chamber social interaction test
  • Heterozygous mice: contain 3 copies of Gldc
    • Behaviorally, mice display a similar phenotype (with no deficit in the water T maze)
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Uwe Rudolph, M.D., University of Illinois at Urbana-Champaign.
Primary Reference

Kambali M, Li Y, Unichenko P, Feria Pliego JA, Yadav R, Liu J, McGuinness P, Cobb JG, Wang M, Nagarajan R, Lyu J, Vongsouthi V, Jackson CJ, Engin E, Coyle JT, Shin J, Hodgson NW, Hensch TK, Talkowski ME, Homanics GE, Bolshakov VY, Henneberger C, Rudolph U. An increased copy number of glycine decarboxylase (GLDC) associated with psychosis reduces extracellular glycine and impairs NMDA receptor function. Mol Psychiatry. 2024 Aug 30. doi: 10.1038/s41380-024-02711-5. Epub ahead of print. (Medline PMID: 39210012)

Strain Development
Two loxP sites flanking the Gldc gene were inserted into the mouse genome, one loxP site by gene targeting in v6.5 ES cells (which the authors report come from 129S1 mice), the other one by CRISPR/Cas-9 in C57BL/6 zygotes. Mice carrying both loxP sites and an Hprt-Cre transgene were generated and bred with C57BL/6J wild type mice. Offspring were screened for the presence of the Gldc duplication. In case the cre transgene was present, it was bred out. Selectable neomycin resistance markers that were included in the targeting vectors have been removed using Flp-FRT recombination. Mice were bred to C57BL/6J wild type mice for at least 8 generations. Mice were then inbred for 5 generations.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
8
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 Weeks
Average Pups Weaned
Undetermined

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Nina Dinjaski.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Nina Dinjaski

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
072077-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
072077-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.